LAPSE:2023.20979
Published Article
LAPSE:2023.20979
Microbial Profile of the Leachate from Mexico City’s Bordo Poniente Composting Plant: An Inoculum to Digest Organic Waste
March 21, 2023
Abstract
In recent years, municipal solid waste (MSW) management has become a complex problem worldwide. Similarly, Mexico City is facing such a situation for the management and treatment of organic fraction of municipal solid waste (OFMSW). Therefore, in this work, we investigated whether leachate from the composting plant, Bordo Poniente, located in Mexico City can be used as an inoculum for the treatment of OFMSW using thermophilic anaerobic digestion (AD) with a hydraulic retention time of 30 days. We analyzed the physicochemical properties of the leachate and performed a biochemical methane potential test. Archaeal and bacterial diversity was also identified using high throughput DNA sequencing of 16S rDNA libraries. Methane yield was 0.29 m3 CH4/kg VSadded in the positive control and 0.16 m3 CH4/kg VSadded in the treatment group. The phylum, Bacteroidetes, and genus, Methanosarcina, prevailed in the leachate. However, in thermophilic conditions, the microbial communities changed, and the phylum, Firmicutes, genera, Methanoculleus, and candidate genus, vadinCA11, were dominant in the treatment group. We concluded that the leachate contains a suitable initial charge of many active bacteria and methanogenic archaea which contribute to the AD process, hence it can be used as an inoculum for the treatment of OFMSW.
In recent years, municipal solid waste (MSW) management has become a complex problem worldwide. Similarly, Mexico City is facing such a situation for the management and treatment of organic fraction of municipal solid waste (OFMSW). Therefore, in this work, we investigated whether leachate from the composting plant, Bordo Poniente, located in Mexico City can be used as an inoculum for the treatment of OFMSW using thermophilic anaerobic digestion (AD) with a hydraulic retention time of 30 days. We analyzed the physicochemical properties of the leachate and performed a biochemical methane potential test. Archaeal and bacterial diversity was also identified using high throughput DNA sequencing of 16S rDNA libraries. Methane yield was 0.29 m3 CH4/kg VSadded in the positive control and 0.16 m3 CH4/kg VSadded in the treatment group. The phylum, Bacteroidetes, and genus, Methanosarcina, prevailed in the leachate. However, in thermophilic conditions, the microbial communities changed, and the phylum, Firmicutes, genera, Methanoculleus, and candidate genus, vadinCA11, were dominant in the treatment group. We concluded that the leachate contains a suitable initial charge of many active bacteria and methanogenic archaea which contribute to the AD process, hence it can be used as an inoculum for the treatment of OFMSW.
Record ID
Keywords
high throughput DNA sequencing, leachate, methanogens, microbial diversity, organic fraction of municipal solid waste, thermophilic anaerobic digestion
Subject
Suggested Citation
Gállego Bravo AK, Salcedo Serrano DA, López Jiménez G, Nirmalkar K, Murugesan S, García-Mena J, Gutiérrez Castillo ME, Tovar Gálvez LR. Microbial Profile of the Leachate from Mexico City’s Bordo Poniente Composting Plant: An Inoculum to Digest Organic Waste. (2023). LAPSE:2023.20979
Author Affiliations
Gállego Bravo AK: Instituto Politécnico Nacional, Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y Desarrollo, Calle 30 de Junio de 1520 s/n, 07340 Ciudad de México, Mexico; Instituto Politécnico Nacional, Centro Mexicano para la Producció
Salcedo Serrano DA: Instituto Politécnico Nacional, Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y Desarrollo, Calle 30 de Junio de 1520 s/n, 07340 Ciudad de México, Mexico; Departamento de Horticultura, Universidad Autónoma Agraria Antonio
López Jiménez G: Departamento de Ciencias Básicas, Instituto Politécnico Nacional, Unidad Profesional Interdisciplinaria de Biotecnología, Av. Acueducto s/n, 07340 Ciudad de México, Mexico
Nirmalkar K: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. Instituto Politécnico Nacional 2508, 07360 Ciudad de México, Mexico [ORCID]
Murugesan S: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. Instituto Politécnico Nacional 2508, 07360 Ciudad de México, Mexico; Division of Translational Medicine, Research Department, Sidra Medicine, [ORCID]
García-Mena J: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. Instituto Politécnico Nacional 2508, 07360 Ciudad de México, Mexico [ORCID]
Gutiérrez Castillo ME: Instituto Politécnico Nacional, Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y Desarrollo, Calle 30 de Junio de 1520 s/n, 07340 Ciudad de México, Mexico
Tovar Gálvez LR: Instituto Politécnico Nacional, Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y Desarrollo, Calle 30 de Junio de 1520 s/n, 07340 Ciudad de México, Mexico
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Salcedo Serrano DA: Instituto Politécnico Nacional, Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y Desarrollo, Calle 30 de Junio de 1520 s/n, 07340 Ciudad de México, Mexico; Departamento de Horticultura, Universidad Autónoma Agraria Antonio
López Jiménez G: Departamento de Ciencias Básicas, Instituto Politécnico Nacional, Unidad Profesional Interdisciplinaria de Biotecnología, Av. Acueducto s/n, 07340 Ciudad de México, Mexico
Nirmalkar K: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. Instituto Politécnico Nacional 2508, 07360 Ciudad de México, Mexico [ORCID]
Murugesan S: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. Instituto Politécnico Nacional 2508, 07360 Ciudad de México, Mexico; Division of Translational Medicine, Research Department, Sidra Medicine, [ORCID]
García-Mena J: Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Av. Instituto Politécnico Nacional 2508, 07360 Ciudad de México, Mexico [ORCID]
Gutiérrez Castillo ME: Instituto Politécnico Nacional, Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y Desarrollo, Calle 30 de Junio de 1520 s/n, 07340 Ciudad de México, Mexico
Tovar Gálvez LR: Instituto Politécnico Nacional, Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y Desarrollo, Calle 30 de Junio de 1520 s/n, 07340 Ciudad de México, Mexico
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Journal Name
Energies
Volume
12
Issue
12
Article Number
E2343
Year
2019
Publication Date
2019-06-19
ISSN
1996-1073
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PII: en12122343, Publication Type: Journal Article
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LAPSE:2023.20979
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https://doi.org/10.3390/en12122343
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