Abstract
Microalgae are considered to be potentially attractive feedstocks for biodiesel production, mainly due to their fast growth rate and high oil content accumulated in their cells. In this study, the suitability for biofuel production was tested for Chlorella vulgaris, Chlorella fusca, Oocystis submarina, and Monoraphidium strain. The effect of nutrient limitation on microalgae biomass growth, lipid accumulation, ash content, fatty acid profile, and selected physico-chemical parameters of algal biodiesel were analysed. The study was carried out in vertical tubular photobioreactors of 100 L capacity. The highest biomass content at 100% medium dose was found for Monoraphidium 525 ± 29 mg·L−1. A 50% reduction of nutrients in the culture medium decreased the biomass content by 23% for O. submarina, 19% for Monoraphidium, 13% for C. vulgaris and 9% for C. fusca strain. Nutrient limitation increased lipid production and reduced ash content in microalgal cells. The highest values were observed for Oocystis submarina, with a 90% increase in lipids and a 45% decrease in ash content in the biomass under stress conditions. The fatty acid profile of particular microalgae strains was dominated by palmitic, oleic, linoleic, and linoleic acids. Nutrient stress increased the amount of saturated and unsaturated fatty acids affecting the quality of biodiesel, but this was determined by the type of strain.