LAPSE:2023.16247
Published Article
LAPSE:2023.16247
Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant Candida tropicalis
March 3, 2023
, a xylose-fermenting yeast, has the potential for converting cellulosic biomass to ethanol. Thermotolerant C. tropicalis X-17, which was isolated in Laos, was subjected to repetitive long-term cultivation with a gradual increase in temperature (RLCGT) in the presence of a high concentration of glucose, which exposed cells to various stresses in addition to the high concentration of glucose and high temperatures. The resultant adapted strain demonstrated increased tolerance to ethanol, furfural and hydroxymethylfurfural at high temperatures and displayed improvement in fermentation ability at high glucose concentrations and xylose-fermenting ability. Transcriptome analysis revealed the up-regulation of a gene for a glucose transporter of the major facilitator superfamily and genes for stress response and cell wall proteins. Additionally, hydropathy analysis revealed that three genes for putative membrane proteins with multiple membrane-spanning segments were also up-regulated. From these findings, it can be inferred that the up-regulation of genes, including the gene for a glucose transporter, is responsible for the phenotype of the adaptive strain. This study revealed part of the mechanisms of fermentability at high glucose concentrations in C. tropicalis and the results of this study suggest that RLCGT is an effective procedure for improving multistress tolerance.
Record ID
Keywords
adaptive laboratory evolution, Candida tropicalis, fermentability at high glucose concentrations, fermenting yeast, multistress tolerance
Subject
Suggested Citation
Phommachan K, Keo-oudone C, Nurcholis M, Vongvilaisak N, Chanhming M, Savanhnaly V, Bounphanmy S, Matsutani M, Kosaka T, Limtong S, Yamada M. Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant Candida tropicalis. (2023). LAPSE:2023.16247
Author Affiliations
Phommachan K: Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan
Keo-oudone C: Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos
Nurcholis M: Department of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University, Malang 65145, Indonesia [ORCID]
Vongvilaisak N: Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos
Chanhming M: Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos
Savanhnaly V: Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos
Bounphanmy S: Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos
Matsutani M: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan
Kosaka T: Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan; Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan; Research Center for Thermotolerant Mic [ORCID]
Limtong S: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand [ORCID]
Yamada M: Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan; Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan; Research Center for Thermotolerant Mic [ORCID]
Keo-oudone C: Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos
Nurcholis M: Department of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University, Malang 65145, Indonesia [ORCID]
Vongvilaisak N: Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos
Chanhming M: Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos
Savanhnaly V: Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos
Bounphanmy S: Department of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, Laos
Matsutani M: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan
Kosaka T: Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan; Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan; Research Center for Thermotolerant Mic [ORCID]
Limtong S: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand [ORCID]
Yamada M: Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan; Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, Japan; Research Center for Thermotolerant Mic [ORCID]
Journal Name
Energies
Volume
15
Issue
2
First Page
561
Year
2022
Publication Date
2022-01-13
Published Version
ISSN
1996-1073
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PII: en15020561, Publication Type: Journal Article
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LAPSE:2023.16247
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doi:10.3390/en15020561
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