How to improve the combustion efficiency and reduce harmful emissions has been a hot research topic in the engine field and related disciplines. Researchers have found that nano-additives to diesel-biodiesel fuel blends have achieved significant results. Many research results and both current and previous studies on nanoparticles have shown that nano-additives play an essential role in improving the performance of internal combustion engines and reducing the emission of harmful substances. This paper summarizes the recent research progress of nanoparticles as additives for diesel-biodiesel fuel blends. Firstly, the excellent properties of nanoparticles are described in detail, and the preparation methods are summarized and discussed. Secondly, the effects of several commonly used nanoparticles as diesel-biodiesel fuel blends on combustion performance and harmful substances emissions in terms of combustion thermal efficiency, brake specific fuel consumption, CO, UHC and NOx, are reviewed. Finally, the effects of nano-additives on internal combustion engines, the environment and human health are discussed. The work carried out in this paper can effectively contribute to the application of nanomaterials in the fuel field. Based on our work, the researchers can efficiently select suitable nano-additives that enable internal combustion engines to achieve efficient combustion and low-emission characteristics.