Deep geothermal energy is a renewable and environmentally friendly resource, and the hot dry rock in a geothermal reservoir is subjected to thermal cycling treatment. Thermal cycling treatment can cause thermal stresses in the rock matrix and result in thermal cracking, which significantly influence the physical and mechanical properties of a rock. To investigate the influence of thermal cycling treatment on the microcrack propagation and mechanical behavior of a granite rock, a series of physical and mechanical tests were performed on nontreated and treated granite samples. The testing results show that the mass, density, and P-wave velocity of granite decrease with heating temperature and cycling time increase, while the volume of the samples increases significantly. The UCS and elastic modulus of the granite declined from 178.65 MPa and 20.09 GPa to 24.58 MPa and 3.81 GPa after treatment at 500 °C for 30 thermal cycling times, respectively. The degradation trends of the UCS and the elastic modulus of the granite can be characterized by the heating temperature and the thermal cycling times. High temperature and frequent thermal cycling treatment can induce microcrack propagation within the granite, which causes the failure of the samples and leads a transformation of granite from brittleness to ductility.