LAPSE:2023.1576
Published Article
LAPSE:2023.1576
Production of Extracellular Lipase by Bacillus halotolerans from Oil-Contaminated Soil in a Pilot-Scale Submerged Bioreactor
February 21, 2023
Microbial lipases are the biocatalyst of choice for the present and future because of their characteristics, including their ability to remain active as an enzyme throughout a broad pH, temperature, and substrate range. The goal of the current investigation was to find novel sources of substrates and isolates from soil contaminated by oil for the synthesis of lipase. On tributyrin media, 10 lipolytic bacterial strains that were isolated from oil-contaminated soil were grown. Using the zone of clearance, it was possible to identify the isolates with the highest activity. Following phylogenetic tree analysis, molecular characterization of the 16S rRNA sequence of the bacterial isolates revealed that it was Bacillus halotolerans (VSH 09). The enzyme was purified to near homogeneity. The enzyme activity was found to be optimum at a pH of 7.0 and a temperature of 35 °C. While Ni2+ and Cu2+ had no effect, the presence of Mg2+ and Ca2+ exhibited the highest levels of enzyme activity. At 1%, tributyrin as a substrate exhibited its highest level of activity. The molecular weight, as determined by SDS-PAGE, was found to be 38 kDa. The kinetics of the enzyme were found to be 41.66 and 9.37 mg/mL for Vmax and Km, respectively. The high yield of lipase produced by this method suggests that it holds potential for production on a large scale and could be used for various biotechnological applications.
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Keywords
Bacillus halotolerans, biocatalyst, bioreactor, environment, lipase
Subject
Suggested Citation
Mahnashi MH, Hombalimath VS, Shaikh IA, Muddapur UM, Desai SV, Achappa S, El-Sherbiny M, Ghoneim MM, Jefri OA, Alshahrani MM, Alhujaily M, Al Ali A, Mannasaheb BA, Al Awadh AA, Khan AA. Production of Extracellular Lipase by Bacillus halotolerans from Oil-Contaminated Soil in a Pilot-Scale Submerged Bioreactor. (2023). LAPSE:2023.1576
Author Affiliations
Mahnashi MH: Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran 66462, Saudi Arabia
Hombalimath VS: Department of Biotechnology, KLE Technological University, Hubballi 580031, Karnataka, India
Shaikh IA: Department of Pharmacology, College of Pharmacy, Najran University, Najran 66462, Saudi Arabia [ORCID]
Muddapur UM: Department of Biotechnology, KLE Technological University, Hubballi 580031, Karnataka, India [ORCID]
Desai SV: Department of Biotechnology, KLE Technological University, Hubballi 580031, Karnataka, India
Achappa S: Department of Biotechnology, KLE Technological University, Hubballi 580031, Karnataka, India [ORCID]
El-Sherbiny M: Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, P.O. Box 71666, Riyadh 11597, Saudi Arabia [ORCID]
Ghoneim MM: Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy, Al-Azhar University, Cairo 11884, Egypt; Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Dariyah 13713, Saudi Arabia [ORCID]
Jefri OA: Department of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia
Alshahrani MM: Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Najran University, 1988, Najran 61441, Saudi Arabia [ORCID]
Alhujaily M: Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, University of Bisha, 255, Al Nakhil, Bisha 67714, Saudi Arabia
Al Ali A: Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, University of Bisha, 255, Al Nakhil, Bisha 67714, Saudi Arabia [ORCID]
Mannasaheb BA: Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Dariyah 13713, Saudi Arabia [ORCID]
Al Awadh AA: Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Najran University, 1988, Najran 61441, Saudi Arabia
Khan AA: Department of General Science, Ibn Sina National College for Medical Studies, Jeddah 21418, Saudi Arabia
Hombalimath VS: Department of Biotechnology, KLE Technological University, Hubballi 580031, Karnataka, India
Shaikh IA: Department of Pharmacology, College of Pharmacy, Najran University, Najran 66462, Saudi Arabia [ORCID]
Muddapur UM: Department of Biotechnology, KLE Technological University, Hubballi 580031, Karnataka, India [ORCID]
Desai SV: Department of Biotechnology, KLE Technological University, Hubballi 580031, Karnataka, India
Achappa S: Department of Biotechnology, KLE Technological University, Hubballi 580031, Karnataka, India [ORCID]
El-Sherbiny M: Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, P.O. Box 71666, Riyadh 11597, Saudi Arabia [ORCID]
Ghoneim MM: Department of Pharmacognosy and Medicinal Plants, Faculty of Pharmacy, Al-Azhar University, Cairo 11884, Egypt; Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Dariyah 13713, Saudi Arabia [ORCID]
Jefri OA: Department of Biological Science, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia
Alshahrani MM: Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Najran University, 1988, Najran 61441, Saudi Arabia [ORCID]
Alhujaily M: Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, University of Bisha, 255, Al Nakhil, Bisha 67714, Saudi Arabia
Al Ali A: Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, University of Bisha, 255, Al Nakhil, Bisha 67714, Saudi Arabia [ORCID]
Mannasaheb BA: Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Dariyah 13713, Saudi Arabia [ORCID]
Al Awadh AA: Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Najran University, 1988, Najran 61441, Saudi Arabia
Khan AA: Department of General Science, Ibn Sina National College for Medical Studies, Jeddah 21418, Saudi Arabia
Journal Name
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Volume
10
Issue
8
First Page
1548
Year
2022
Publication Date
2022-08-07
Published Version
ISSN
2227-9717
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PII: pr10081548, Publication Type: Journal Article
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LAPSE:2023.1576
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doi:10.3390/pr10081548
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Feb 21, 2023
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