This paper analyzes the results of a trial operation of a battery-based buffer station supporting a selected section of trolleybus power supply systems in Pilsen, Czech Republic. The buffer station aims to prevent the catenary from excessive voltage drops in a part of the route that is most remote from the traction substation. Compensation of voltage drops is carried out by continuously measuring the catenary voltage and injecting the current into the catenary if the voltage decreases below a preset value. The effectiveness of such a solution was evaluated by the analysis of numerous experimental recordings, both from the buffer station itself and from trolleybuses in operation. Further on, based on the recordings, a utilized battery capacity was estimated and a control method for decreasing the required capacity was proposed. The optimal capacity analysis, supplemented with an evaluation of required output current rating, was used to define the best storage technology for the considered application.