In the study, rupture energy values of Deveci and Abate Fetel pear fruits were predicted using artificial neural network (ANN). This research aimed to develop a simple, accurate, rapid, and economic model for harvest/post-harvest loss of efficiently predicting rupture energy values of Deveci and Abate Fetel pear fruits. The breaking energy of the pears was examined in terms of storage time and loading position. The experiments were carried out in two stages, with samples kept in cold storage immediately after harvest and 30 days later. Rupture energy values were estimated using four different single and multi-layer ANN models. Four different model results obtained using Levenberg−Marquardt, Scaled Conjugate Gradient, and resilient backpropagation training algorithms were compared with the calculated values. Statistical parameters such as R2, RMSE, MAE, and MSE were used to evaluate the performance of the methods. The best-performing model was obtained in network structure 5-1 that used three inputs: the highest R2 value (0.90) and the lowest square of the root error (0.018), and the MAE (0.093).