Cumulative Production of Bioactive Rg3, Rg5, Rk1, and CK from Fermented Black Ginseng Using Novel Aspergillus niger KHNT-1 Strain Isolated from Korean Traditional Food

Jin Kyu Park; Dong Uk Yang; Lakshminarayanan Arunkumar; Yaxi Han; Seung Jin Lee; Muhammad Huzaifa Arif; Jin Feng Li; Yue Huo; Jong Pyo Kang; Van An Hoang; Jong Chan Ahn; Deok Chun Yang; Se Chan Kang

LAPSE

Living Archive for Process Systems Engineering

LAPSE:2022.0068

Published Article

LAPSE:2022.0068

Cumulative Production of Bioactive Rg3, Rg5, Rk1, and CK from Fermented Black Ginseng Using Novel Aspergillus niger KHNT-1 Strain Isolated from Korean Traditional Food

Jin Kyu Park, Dong Uk Yang, Lakshminarayanan Arunkumar, Yaxi Han, Seung Jin Lee, Muhammad Huzaifa Arif, Jin Feng Li, Yue Huo, Jong Pyo Kang, Van An Hoang, Jong Chan Ahn, Deok Chun Yang, Se Chan Kang

October 13, 2022

Ginseng is an ancient herb widely consumed due to its healing property of active ginsenosides. Recent researchers were explored to increase its absorption and bioavailability of ginsenosides at the metabolic sites, due to its pharmacological activity. The purpose of this study was to investigate the isolation and characteristics of components obtained by a shorter steaming cycle (seven cycles) of white ginseng to fermented black ginseng, using a novel strain of Aspergillus niger KHNT-1 isolated from fermented soybean. The degree of bioactive of Rg3 increased effectively during the steaming process, and biotransformation converted the color towards black along active ginsenosides. Glycol moiety associated with C-3, C-6, or C-20 underwent rapid biotransformation and hydrolysis, such as Rb1, Rb2, Rc, Rd → Rg3, F2, and was converted to CK. Dehydration produces Rg3 → Rk1, Rg5. Rh2 → Rk2; thus, converted fermented black ginseng was solvent-extracted, and the isolated components were identified by TLC, HPLC, and quantification by LCMS. The unique composition obtained during this process with Rk1, Rg3, Rg5, and CK is nontoxic to HaCaT cell line up to 200 ug/mL for 24 h and was found to be effective in B16BL6 cell lines, in a dose- and time-dependent manner. Thus, it is a suitable candidate for nutraceuticals and cosmeceuticals.

Record ID

LAPSE:2022.0068

Keywords

Aspergillus niger, B16BL6 (Murine melanoma) cell line, ginseng, Ginseng Lateral root (GLR), Ginseng Main root (GMR), ginsenosides, human keratinocyte cell line (HaCaT) cells, processing, soybean

Subject

Food & Agricultural Processes

Suggested Citation

Park JK, Yang DU, Arunkumar L, Han Y, Lee SJ, Arif MH, Li JF, Huo Y, Kang JP, Hoang VA, Ahn JC, Yang DC, Kang SC. Cumulative Production of Bioactive Rg3, Rg5, Rk1, and CK from Fermented Black Ginseng Using Novel Aspergillus niger KHNT-1 Strain Isolated from Korean Traditional Food. (2022). LAPSE:2022.0068

Author Affiliations

Park JK: Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea
Yang DU: Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea
Arunkumar L: Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea
Han Y: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea
Lee SJ: Nature Bio Pharma Co., Ltd., Gangnam-gu, Seoul 06241, Korea
Arif MH: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea
Li JF: Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea
Huo Y: Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea
Kang JP: Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea [ORCID]
Hoang VA: Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea
Ahn JC: Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea
Yang DC: Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea; Department of Oriental Medicinal Biotechnology, College of Life Science, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea [ORCID]
Kang SC: Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Korea [ORCID]

Journal Name

Processes

Volume

9

Issue

2

First Page

227

Year

2021

Publication Date

2021-01-26