LAPSE:2021.0704
Published Article
LAPSE:2021.0704
Quantitative Determination of Vitamins A and E in Ointments Using Raman Spectroscopy
Sylwester Mazurek, Kamil Pichlak, Roman Szostak
August 2, 2021
A quantitative analysis of vitamins A and E in commercial ointments containing 0.044% and 0.8% (w/w) of active pharmaceutical ingredients, respectively, was performed using partial least squares models based on FT Raman spectra. Separate calibration systems were prepared to determine the amount of vitamin A in a petrolatum base ointment and to quantify vitamins A and E in a eucerin base one. Compositions of the laboratory-prepared and commercial samples were controlled through a principal component analysis. Relative standard errors of prediction were calculated to compare the predictive ability of the obtained regression models. For vitamin A determination, these errors were found to be in the 3.8−5.0% and 5.7−5.9% ranges for the calibration and validation data sets, respectively. In the case of vitamin E modeling, these errors amounted to 3.7% and 4.4%. On the basis of elaborated models, vitamins A and E were successfully quantified in two commercial products with recoveries in the 99−104% range. The obtained data indicate that the Raman technique allows for accurate analysis of the composition of semisolid formulations in their native state, including low dose preparations.
Keywords
chemometrics, multivariate calibration, ointments, quantitative analysis, Raman spectroscopy, vitamin A, vitamin E
Suggested Citation
Mazurek S, Pichlak K, Szostak R. Quantitative Determination of Vitamins A and E in Ointments Using Raman Spectroscopy. (2021). LAPSE:2021.0704
Author Affiliations
Mazurek S: Laboratory of Chemometrics and Applied Spectroscopy, Department of Chemistry, University of Wrocław, 14 F. Joliot-Curie, 50-383 Wrocław, Poland
Pichlak K: Laboratory of Chemometrics and Applied Spectroscopy, Department of Chemistry, University of Wrocław, 14 F. Joliot-Curie, 50-383 Wrocław, Poland
Szostak R: Laboratory of Chemometrics and Applied Spectroscopy, Department of Chemistry, University of Wrocław, 14 F. Joliot-Curie, 50-383 Wrocław, Poland [ORCID]
Journal Name
Processes
Volume
9
Issue
1
First Page
pr9010008
Year
2020
Publication Date
2020-12-23
ISSN
2227-9717
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Original Submission
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PII: pr9010008, Publication Type: Journal Article
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LAPSE:2021.0704
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https://doi.org/10.3390/pr9010008
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Aug 2, 2021
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Calvin Tsay
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