LAPSE:2021.0683v1
Published Article
LAPSE:2021.0683v1
VectorDisk: A Microfluidic Platform Integrating Diagnostic Markers for Evidence-Based Mosquito Control
July 29, 2021
Effective mosquito monitoring relies on the accurate identification and characterization of the target population. Since this process requires specialist knowledge and equipment that is not widely available, automated field-deployable systems are highly desirable. We present a centrifugal microfluidic cartridge, the VectorDisk, which integrates TaqMan PCR assays in two feasibility studies, aiming to assess multiplexing capability, specificity, and reproducibility in detecting disk-integrated vector-related assays. In the first study, pools of 10 mosquitoes were used as samples. We tested 18 disks with 27 DNA and RNA assays each, using a combination of multiple microfluidic chambers and detection wavelengths (geometric and color multiplexing) to identify mosquito and malaria parasite species as well as insecticide resistance mechanisms. In the second study, purified nucleic acids served as samples to test arboviral and malaria infective mosquito assays. Nine disks were tested with 14 assays each. No false positive results were detected on any of the disks. The coefficient of variation in reproducibility tests was <10%. The modular nature of the platform, the easy adaptation of the primer/probe panels, the cold chain independence, the rapid (2−3 h) analysis, and the assay multiplexing capacity are key features, rendering the VectorDisk a potential candidate for automated vector analysis.
Record ID
Keywords
arboviruses, insecticide resistances, LabDisk, malaria, microfluidics, mosquito monitoring, vector-borne diseases
Subject
Suggested Citation
Hin S, Baumgartner D, Specht M, Lüddecke J, Mahmodi Arjmand E, Johannsen B, Schiedel L, Rombach M, Paust N, von Stetten F, Zengerle R, Wipf N, Müller P, Mavridis K, Vontas J, Mitsakakis K. VectorDisk: A Microfluidic Platform Integrating Diagnostic Markers for Evidence-Based Mosquito Control. (2021). LAPSE:2021.0683v1
Author Affiliations
Hin S: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Baumgartner D: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; Laboratory for MEMS Applications, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Specht M: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Lüddecke J: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Mahmodi Arjmand E: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Johannsen B: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Schiedel L: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Rombach M: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Paust N: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; Laboratory for MEMS Applications, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
von Stetten F: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; Laboratory for MEMS Applications, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Zengerle R: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; Laboratory for MEMS Applications, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Wipf N: Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002 Basel, Switzerland; University of Basel, Petersplatz 1, 4001 Basel, Switzerland [ORCID]
Müller P: Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002 Basel, Switzerland; University of Basel, Petersplatz 1, 4001 Basel, Switzerland [ORCID]
Mavridis K: Foundation for Research and Technology-Hellas, Institute of Molecular Biology and Biotechnology, 70013 Heraklion, Greece [ORCID]
Vontas J: Foundation for Research and Technology-Hellas, Institute of Molecular Biology and Biotechnology, 70013 Heraklion, Greece; Pesticide Science Laboratory, Department of Crop Science, Agricultural University of Athens, 11855 Athens, Greece
Mitsakakis K: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; Laboratory for MEMS Applications, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Baumgartner D: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; Laboratory for MEMS Applications, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Specht M: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Lüddecke J: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Mahmodi Arjmand E: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Johannsen B: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Schiedel L: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Rombach M: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Paust N: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; Laboratory for MEMS Applications, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
von Stetten F: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; Laboratory for MEMS Applications, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Zengerle R: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; Laboratory for MEMS Applications, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Wipf N: Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002 Basel, Switzerland; University of Basel, Petersplatz 1, 4001 Basel, Switzerland [ORCID]
Müller P: Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002 Basel, Switzerland; University of Basel, Petersplatz 1, 4001 Basel, Switzerland [ORCID]
Mavridis K: Foundation for Research and Technology-Hellas, Institute of Molecular Biology and Biotechnology, 70013 Heraklion, Greece [ORCID]
Vontas J: Foundation for Research and Technology-Hellas, Institute of Molecular Biology and Biotechnology, 70013 Heraklion, Greece; Pesticide Science Laboratory, Department of Crop Science, Agricultural University of Athens, 11855 Athens, Greece
Mitsakakis K: Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; Laboratory for MEMS Applications, IMTEK—Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany
Journal Name
Processes
Volume
8
Issue
12
Article Number
E1677
Year
2020
Publication Date
2020-12-18
ISSN
2227-9717
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PII: pr8121677, Publication Type: Journal Article
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LAPSE:2021.0683v1
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https://doi.org/10.3390/pr8121677
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Jul 29, 2021
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Record Owner
Calvin Tsay
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