LAPSE:2021.0350v1
Published Article
LAPSE:2021.0350v1
Bacterial Toxicity Testing: Modification and Evaluation of the Luminescent Bacteria Test and the Respiration Inhibition Test
May 11, 2021
The activated sludge respiration inhibition test and the luminescent bacteria test with Vibrio fischeri are important bacterial test systems for evaluation of the toxicity of chemical compounds. These test systems were further optimized to result in better handling, reliability and sensitivity. Concerning the Vibrio fischeri test, media components such as yeast extract and bivalent cation concentrations like Ca2+ and Mg2+ were optimized. The cultivation, storage conditions and reactivation process of the stored bacteria were also improved, which enabled simpler handling and led to good reproducibility. Additionally, the respiration inhibition test with a prolonged incubation time was further analyzed using different chlorinated phenols as reference compounds. It could be stated that a longer incubation period significantly improved the sensitivity of the test system.
Record ID
Keywords
activated sludge, bacterial toxicity, chlorinated phenols, Cr(VI) and Zn(II) cations, long-term toxicity, luminescent bacteria, respiration inhibition
Subject
Suggested Citation
Strotmann U, Pastor Flores D, Konrad O, Gendig C. Bacterial Toxicity Testing: Modification and Evaluation of the Luminescent Bacteria Test and the Respiration Inhibition Test. (2021). LAPSE:2021.0350v1
Author Affiliations
Strotmann U: Department of Chemistry, Westfälische Hochschule, University of Applied Sciences, August-Schmidt-Ring 10, 45665 Recklinghausen, Germany [ORCID]
Pastor Flores D: Department of Chemistry, Westfälische Hochschule, University of Applied Sciences, August-Schmidt-Ring 10, 45665 Recklinghausen, Germany; KBI Biopharma BVBA, Technologielaan 8, 3001 Leuven, Belgium
Konrad O: Laboratory of Bioreactors, Univates University, Rua Avelino Tallini, 171, 95900 Lajeado/RS, Brazil
Gendig C: Department of Chemistry, Westfälische Hochschule, University of Applied Sciences, August-Schmidt-Ring 10, 45665 Recklinghausen, Germany
Pastor Flores D: Department of Chemistry, Westfälische Hochschule, University of Applied Sciences, August-Schmidt-Ring 10, 45665 Recklinghausen, Germany; KBI Biopharma BVBA, Technologielaan 8, 3001 Leuven, Belgium
Konrad O: Laboratory of Bioreactors, Univates University, Rua Avelino Tallini, 171, 95900 Lajeado/RS, Brazil
Gendig C: Department of Chemistry, Westfälische Hochschule, University of Applied Sciences, August-Schmidt-Ring 10, 45665 Recklinghausen, Germany
Journal Name
Processes
Volume
8
Issue
11
Article Number
E1349
Year
2020
Publication Date
2020-10-25
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr8111349, Publication Type: Journal Article
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Published Article
LAPSE:2021.0350v1
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External Link
https://doi.org/10.3390/pr8111349
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[v1] (Original Submission)
May 11, 2021
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May 11, 2021
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v1
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https://psecommunity.org/LAPSE:2021.0350v1
Record Owner
Calvin Tsay
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