LAPSE:2020.1199
Published Article
LAPSE:2020.1199
Electrophysiological Effects of Extracellular Vesicles Secreted by Cardiosphere-Derived Cells: Unraveling the Antiarrhythmic Properties of Cell Therapies
December 17, 2020
Although cell-based therapies show potential antiarrhythmic effects that could be mediated by their paracrine action, the mechanisms and the extent of these effects were not deeply explored. We investigated the antiarrhythmic mechanisms of extracellular vesicles secreted by cardiosphere-derived cell extracellular vesicles (CDC-EVs) on the electrophysiological properties and gene expression profile of HL1 cardiomyocytes. HL-1 cultures were primed with CDC-EVs or serum-free medium alone for 48 h, followed by optical mapping and gene expression analysis. In optical mapping recordings, CDC-EVs reduced the activation complexity of the cardiomyocytes by 40%, increased rotor meandering, and reduced rotor curvature, as well as induced an 80% increase in conduction velocity. HL-1 cells primed with CDC-EVs presented higher expression of SCN5A, CACNA1C, and GJA1, coding for proteins involved in INa, ICaL, and Cx43, respectively. Our results suggest that CDC-EVs reduce activation complexity by increasing conduction velocity and modifying rotor dynamics, which could be driven by an increase in expression of SCN5A and CACNA1C genes, respectively. Our results provide new insights into the antiarrhythmic mechanisms of cell therapies, which should be further validated using other models.
Record ID
Keywords
antiarrhythmic effects, cell therapy, conduction velocity, extracellular vesicles, optical mapping
Subject
Suggested Citation
Gómez-Cid L, Moro-López M, de la Nava AS, Hernández-Romero I, Fernández AI, Suárez-Sancho S, Atienza F, Grigorian-Shamagian L, Fernández-Avilés F. Electrophysiological Effects of Extracellular Vesicles Secreted by Cardiosphere-Derived Cells: Unraveling the Antiarrhythmic Properties of Cell Therapies. (2020). LAPSE:2020.1199
Author Affiliations
Gómez-Cid L: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 28911 Leganés, Spain; CIBERCV, ISCIII, 28029 Madrid,
Moro-López M: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 28911 Leganés, Spain
de la Nava AS: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; CIBERCV, ISCIII, 28029 Madrid, Spain; ITACA Institute, Universitat Politècnica de València, 46022 Valencia, Spain
Hernández-Romero I: Department of Signal Theory and Communications, Universidad Rey Juan Carlos, 28943 Fuenlabrada, Spain [ORCID]
Fernández AI: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; CIBERCV, ISCIII, 28029 Madrid, Spain
Suárez-Sancho S: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; CIBERCV, ISCIII, 28029 Madrid, Spain
Atienza F: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; CIBERCV, ISCIII, 28029 Madrid, Spain; Faculty of Medicine, Universidad Complutense de Madrid, 28040 Madrid, Spain
Grigorian-Shamagian L: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; CIBERCV, ISCIII, 28029 Madrid, Spain
Fernández-Avilés F: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; CIBERCV, ISCIII, 28029 Madrid, Spain; Faculty of Medicine, Universidad Complutense de Madrid, 28040 Madrid, Spain
Moro-López M: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 28911 Leganés, Spain
de la Nava AS: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; CIBERCV, ISCIII, 28029 Madrid, Spain; ITACA Institute, Universitat Politècnica de València, 46022 Valencia, Spain
Hernández-Romero I: Department of Signal Theory and Communications, Universidad Rey Juan Carlos, 28943 Fuenlabrada, Spain [ORCID]
Fernández AI: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; CIBERCV, ISCIII, 28029 Madrid, Spain
Suárez-Sancho S: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; CIBERCV, ISCIII, 28029 Madrid, Spain
Atienza F: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; CIBERCV, ISCIII, 28029 Madrid, Spain; Faculty of Medicine, Universidad Complutense de Madrid, 28040 Madrid, Spain
Grigorian-Shamagian L: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; CIBERCV, ISCIII, 28029 Madrid, Spain
Fernández-Avilés F: Instituto de Investigación Sanitaria Gregorio Marañón, Hospital Gregorio Marañón, 28009 Madrid, Spain; CIBERCV, ISCIII, 28029 Madrid, Spain; Faculty of Medicine, Universidad Complutense de Madrid, 28040 Madrid, Spain
Journal Name
Processes
Volume
8
Issue
8
Article Number
E924
Year
2020
Publication Date
2020-08-02
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr8080924, Publication Type: Journal Article
Record Map
Published Article
LAPSE:2020.1199
This Record
External Link
doi:10.3390/pr8080924
Publisher Version
Download
Meta
Record Statistics
Record Views
325
Version History
[v1] (Original Submission)
Dec 17, 2020
Verified by curator on
Dec 17, 2020
This Version Number
v1
Citations
Most Recent
This Version
URL Here
https://psecommunity.org/LAPSE:2020.1199
Original Submitter
Calvin Tsay
Links to Related Works