LAPSE:2020.1177
Published Article
LAPSE:2020.1177
Potential Cultivation of Lactobacillus pentosus from Human Breastmilk with Rapid Monitoring through the Spectrophotometer Method
December 17, 2020
The present study focused on the development of a new method to determine the lag phase of Lactobacillus in breast milk which was attained during the 1st, 3rd, and 6th month (M1, M3, and M6). The colonies’ phylogenetic analysis, derived from the 16S rRNA gene sequences, was evaluated with genus Lactobacillus pentosus and achieved a similarity value of 99%. Raman spectroscopy in optical densities of 600 nm (OD600) were used for six consecutive days to observe the changes of the cell growth rate. The values of OD600 were well fitted with the regression model. From this work, M1 was found to be the longest lag phase in 18 h, and it was 17% to 27% longer compared to M3 and M6, respectively. However, the samples of M3 and M6 showed the shortest duration in reaching 0.5 of OD600 nm (16 h) which was enhanced by 80% and 96% compared to M1, respectively. These studies will be of significance when applied in determining the bacteria growth curve and in assessing the growth behavior for the strain in human breast milk.
Record ID
Keywords
bacteria sequencing, breast milk, Lactobacillus isolation, lag phase
Subject
Suggested Citation
Nguyen-Sy T, Yew GY, Chew KW, Nguyen TDP, Tran TNT, Le TDH, Vo CT, Tran HKP, Mubashir M, Show PL. Potential Cultivation of Lactobacillus pentosus from Human Breastmilk with Rapid Monitoring through the Spectrophotometer Method. (2020). LAPSE:2020.1177
Author Affiliations
Nguyen-Sy T: Department of Chemical Engineering and Environment, University of Technology and Education-The University of Danang, 48 Cao Thang St., Danang 550000, Vietnam [ORCID]
Yew GY: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia
Chew KW: School of Energy and Chemical Engineering, Xiamen University Malaysia, Sepang 43900, Selangor Darul Ehsan, Malaysia [ORCID]
Nguyen TDP: Department of Chemical Engineering and Environment, University of Technology and Education-The University of Danang, 48 Cao Thang St., Danang 550000, Vietnam [ORCID]
Tran TNT: Department of Chemical Engineering and Environment, University of Technology and Education-The University of Danang, 48 Cao Thang St., Danang 550000, Vietnam
Le TDH: Department of Chemical Engineering and Environment, University of Technology and Education-The University of Danang, 48 Cao Thang St., Danang 550000, Vietnam
Vo CT: Department of Biology and Environmental Science, The Danang University of Science and Education, 459 Ton Duc Thang St., Danang 550000, Vietnam
Tran HKP: Le Quy Don High School for the Gifted Danang, Danang 550000, Vietnam
Mubashir M: Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands
Show PL: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia [ORCID]
Yew GY: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia
Chew KW: School of Energy and Chemical Engineering, Xiamen University Malaysia, Sepang 43900, Selangor Darul Ehsan, Malaysia [ORCID]
Nguyen TDP: Department of Chemical Engineering and Environment, University of Technology and Education-The University of Danang, 48 Cao Thang St., Danang 550000, Vietnam [ORCID]
Tran TNT: Department of Chemical Engineering and Environment, University of Technology and Education-The University of Danang, 48 Cao Thang St., Danang 550000, Vietnam
Le TDH: Department of Chemical Engineering and Environment, University of Technology and Education-The University of Danang, 48 Cao Thang St., Danang 550000, Vietnam
Vo CT: Department of Biology and Environmental Science, The Danang University of Science and Education, 459 Ton Duc Thang St., Danang 550000, Vietnam
Tran HKP: Le Quy Don High School for the Gifted Danang, Danang 550000, Vietnam
Mubashir M: Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, 5600 MB Eindhoven, The Netherlands
Show PL: Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia [ORCID]
Journal Name
Processes
Volume
8
Issue
8
Article Number
E902
Year
2020
Publication Date
2020-07-29
Published Version
ISSN
2227-9717
Version Comments
Original Submission
Other Meta
PII: pr8080902, Publication Type: Journal Article
Record Map
Published Article
LAPSE:2020.1177
This Record
External Link
doi:10.3390/pr8080902
Publisher Version
Download
Meta
Record Statistics
Record Views
343
Version History
[v1] (Original Submission)
Dec 17, 2020
Verified by curator on
Dec 17, 2020
This Version Number
v1
Citations
Most Recent
This Version
URL Here
https://psecommunity.org/LAPSE:2020.1177
Original Submitter
Calvin Tsay
Links to Related Works