LAPSE:2024.1866
Published Article
LAPSE:2024.1866
Cloning, Expression and Enzymatic Characterization of Pectin Methyl Esterase from Populus trichocarpa and Its Application
August 23, 2024
Abstract
The pectin methyl esterase gene from Populus trichocarpa (PtPME) was successfully cloned through PCR amplification and subsequently inserted into the expressing vector pMAL-c5e for successful expression in Escherichia coli BL21 (DE3). Initially, we determined the primary enzymatic properties of PtPME, a pectin methyl esterase derived from Populus trichocarpa. Notably, this enzyme exhibits a higher affinity towards citrus pectin, with an esterification degree exceeding 60%. Furthermore, this enzyme’s optimal reaction temperature and pH were found to be 30 °C and 8, respectively. Importantly, its exceptional stability under neutral conditions highlights its potential application in the industrial production of low-ester pectin.
The pectin methyl esterase gene from Populus trichocarpa (PtPME) was successfully cloned through PCR amplification and subsequently inserted into the expressing vector pMAL-c5e for successful expression in Escherichia coli BL21 (DE3). Initially, we determined the primary enzymatic properties of PtPME, a pectin methyl esterase derived from Populus trichocarpa. Notably, this enzyme exhibits a higher affinity towards citrus pectin, with an esterification degree exceeding 60%. Furthermore, this enzyme’s optimal reaction temperature and pH were found to be 30 °C and 8, respectively. Importantly, its exceptional stability under neutral conditions highlights its potential application in the industrial production of low-ester pectin.
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Keywords
enzymatic characterization, molecular cloning, pectin methyl esterase, Populus trichocarpa
Subject
Suggested Citation
Feng Y, Huang L, Zeng Y, Zhang Y, Liu W, He G. Cloning, Expression and Enzymatic Characterization of Pectin Methyl Esterase from Populus trichocarpa and Its Application. (2024). LAPSE:2024.1866
Author Affiliations
Feng Y: Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, School of Pharmacy, Chengdu University, Chengdu 610106, China
Huang L: Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, School of Pharmacy, Chengdu University, Chengdu 610106, China
Zeng Y: Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, School of Pharmacy, Chengdu University, Chengdu 610106, China
Zhang Y: Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, School of Pharmacy, Chengdu University, Chengdu 610106, China
Liu W: Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, School of Pharmacy, Chengdu University, Chengdu 610106, China; School of Life Science, Leshan Normal University, Leshan 614000, China
He G: Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, School of Pharmacy, Chengdu University, Chengdu 610106, China; State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 10 [ORCID]
Huang L: Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, School of Pharmacy, Chengdu University, Chengdu 610106, China
Zeng Y: Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, School of Pharmacy, Chengdu University, Chengdu 610106, China
Zhang Y: Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, School of Pharmacy, Chengdu University, Chengdu 610106, China
Liu W: Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, School of Pharmacy, Chengdu University, Chengdu 610106, China; School of Life Science, Leshan Normal University, Leshan 614000, China
He G: Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, School of Pharmacy, Chengdu University, Chengdu 610106, China; State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 10 [ORCID]
Journal Name
Processes
Volume
12
Issue
7
First Page
1511
Year
2024
Publication Date
2024-07-18
ISSN
2227-9717
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PII: pr12071511, Publication Type: Journal Article
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LAPSE:2024.1866
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https://doi.org/10.3390/pr12071511
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[v1] (Original Submission)
Aug 23, 2024
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Aug 23, 2024
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