In order to overcome the difficulty of parameter tuning caused by the large lag and time-varying nonlinearity of the tank for lysozyme fermentation, a temperature control method based on LRWOA-LSTM-PID is proposed in this paper. Firstly, according to the intrinsic mechanism of the fermenter, a temperature mechanism model based on a dynamic equation is designed, which can better reflect the temperature changes in the fermenter. Secondly, a Proportional Integral Derivative (PID) parameter tuning method based on a Long-Short Term Memory Network (LSTM) is proposed, which takes advantage of the ability of LSTM to learn time sequence information and obtains the variation trend between error sequences under continuous time sampling, thereby adjusting network weights more reasonably and accelerating PID parameter tuning. Finally, a Whale Optimization Algorithm (WOA) based on the Lévy flight and random walk strategy (LRWOA) is proposed for the initialization of LSTM parameters; this algorithm has excellent optimization capabilities and overcomes the problem of LSTM falling into local optimal solutions prematurely during parameter randomization. The results show that the method proposed in this paper can achieve rapid tuning of PID parameters, thereby improving the convergence speed of the system and reducing system overshoot.