Non-road sectors, such as agriculture and construction machinery, require high energy densities and flexibility in use, which is why diesel engines are mainly used. The use of climate-neutral fuels, produced from renewable energies, such as Oxymethylene Ether (OME) as a diesel substitute, can significantly reduce CO2 and pollutant emissions in these sectors. In addition to CO2 neutrality, OME also offers improved combustion characteristics compared to diesel fuel, eliminating the soot−NOx trade-off and thus enabling new opportunities in engine design and calibration. In this paper, the combustion of pure OME on a close-to-production, single-cylinder non-road diesel engine with a pump−line−nozzle injection system is analyzed. A variation of the center of combustion at constant power output was performed for diesel and OME at different operating points. Two injectors were investigated with OME. A study on ignition delay and a detailed thermodynamic analysis was carried out. In addition, the exhaust emissions CO, NOx, VOC, as well as particulate-matter, -number and -size distributions were measured. With OME, a significantly shorter ignition delay as well as a shortened combustion duration could be observed, despite a longer injection duration. In addition, the maximum injection pressure increases. VOC and CO emissions are reduced. Particulate matter was reduced by more than 99% and particle number (>10 nm) was reduced by multiple orders of magnitude. The median of the particle size distribution shifts from 60 to 85 nm (diesel) into a diameter range of sub 23 nm (OME). A significant reduction of NOx emissions with OME enables new degrees of freedom in engine calibration and an efficiency advantage without hardware adaption.