LAPSE:2023.5644
Published Article
LAPSE:2023.5644
Liposomes with Caffeic Acid: Morphological and Structural Characterisation, Their Properties and Stability in Time
February 23, 2023
Abstract
Medical and pharmaceutical research has shown that liposomes are very efficient in transporting drugs to targets. In this study, we prepared six liposome formulas, three in which we entrapped caffeic acid (CA), and three with only phospholipids and without CA. Determination of entrapment efficiency (EE) showed that regardless of the phospholipids used, the percentage of CA entrapment was up to 76%. The characterization of the liposomes was performed using Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM), zeta potential and polydispersity and showed that about 75−99% of the liposomes had dimensions between 40 ± 0.55−500 ± 1.45 nm. The size and zeta potential of liposomes were influenced by the type of phospholipid used to obtain them. CA release from liposomes was performed using a six-cell Franz diffusion system, and it was observed that the release of entrapped CA occurs gradually, the highest amount occurring in the first eight hours (over 80%), after which the release is much reduced. Additionally, the time stability of the obtained liposomes was analysed using univariate and multivariate statistical analysis. Therefore, liposomes offer great potential in CA entrapment.
Medical and pharmaceutical research has shown that liposomes are very efficient in transporting drugs to targets. In this study, we prepared six liposome formulas, three in which we entrapped caffeic acid (CA), and three with only phospholipids and without CA. Determination of entrapment efficiency (EE) showed that regardless of the phospholipids used, the percentage of CA entrapment was up to 76%. The characterization of the liposomes was performed using Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM), zeta potential and polydispersity and showed that about 75−99% of the liposomes had dimensions between 40 ± 0.55−500 ± 1.45 nm. The size and zeta potential of liposomes were influenced by the type of phospholipid used to obtain them. CA release from liposomes was performed using a six-cell Franz diffusion system, and it was observed that the release of entrapped CA occurs gradually, the highest amount occurring in the first eight hours (over 80%), after which the release is much reduced. Additionally, the time stability of the obtained liposomes was analysed using univariate and multivariate statistical analysis. Therefore, liposomes offer great potential in CA entrapment.
Record ID
Keywords
Atomic Force Microscopy, caffeic acid, Dynamic Light Scattering, liposomes, stability
Suggested Citation
Dejeu IL, Vicaș LG, Jurca T, Teușdea AC, Mureșan ME, Fritea L, Svera P, Gabor GA, Dejeu GE, Maghiar OA, Bodea AS, Pallag A, Marian E. Liposomes with Caffeic Acid: Morphological and Structural Characterisation, Their Properties and Stability in Time. (2023). LAPSE:2023.5644
Author Affiliations
Dejeu IL: Doctoral School of Biomedical Science, University of Oradea, 1 University Street, 410087 Oradea, Romania
Vicaș LG: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania [ORCID]
Jurca T: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Teușdea AC: Faculty of Environmental Protection, University of Oradea, 26th Gen. Magheru Avenue, 410087 Oradea, Romania
Mureșan ME: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Fritea L: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Svera P: INCEMC-National Institute for Research and Development in Electrochemistry and Condensed Matter—Timisoara, No.144 Dr. A. Paunescu Podeanu Street, 300569 Timisoara, Romania
Gabor GA: Faculty of Electrical Engineering and Information Technology, University of Oradea, 1 University Street, 410087 Oradea, Romania
Dejeu GE: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Maghiar OA: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Bodea AS: Doctoral School of Biomedical Science, University of Oradea, 1 University Street, 410087 Oradea, Romania
Pallag A: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania [ORCID]
Marian E: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Vicaș LG: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania [ORCID]
Jurca T: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Teușdea AC: Faculty of Environmental Protection, University of Oradea, 26th Gen. Magheru Avenue, 410087 Oradea, Romania
Mureșan ME: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Fritea L: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Svera P: INCEMC-National Institute for Research and Development in Electrochemistry and Condensed Matter—Timisoara, No.144 Dr. A. Paunescu Podeanu Street, 300569 Timisoara, Romania
Gabor GA: Faculty of Electrical Engineering and Information Technology, University of Oradea, 1 University Street, 410087 Oradea, Romania
Dejeu GE: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Maghiar OA: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Bodea AS: Doctoral School of Biomedical Science, University of Oradea, 1 University Street, 410087 Oradea, Romania
Pallag A: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania [ORCID]
Marian E: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Journal Name
Processes
Volume
9
Issue
6
First Page
912
Year
2021
Publication Date
2021-05-22
ISSN
2227-9717
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Original Submission
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PII: pr9060912, Publication Type: Journal Article
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LAPSE:2023.5644
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https://doi.org/10.3390/pr9060912
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Feb 23, 2023
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