The size effect has a significant effect on the mechanical behavior of rock, thereby fundamentally influencing the stability of rock excavations. The main challenge associated with the experimental research on the size effect of fractured rock mass lies in the difficulty of specimen preparation to represent the influence of size and fracture on the mechanical behavior of the rock material. In order to preliminarily explore the feasibility of 3D printing technology in the field of rock mechanics, fractured rock specimens of different sizes and different fracture characteristics were produced using sand powder 3D printing technology. The uniaxial compression test was combined with the digital image correlation method (DIC) technology to study the influence of the size effect on the mechanical properties and deformation and failure of different fractured specimens. The research finds that: (1) The elastoplastic mechanical characteristics of the sand powder 3D printed specimens are similar to soft rock. Specimen size and fracture angle have significant effects on the mechanical properties of specimens. Under different fracture conditions, the uniaxial compressive strength (UCS) and Elasticity Modulus of sand powder 3D specimens should be decreased with the increase of the specimen size, and the size effect has different influences on the specimens with different fracture characteristics. (2) Under different fracture conditions, the crack initiation position and failure mode of specimens of various sizes are affected by the fracture inclination to varying degrees. (3) The size effect of fractured rock mass is closely related to the defect level inside the rock mass. The size effect originates from the heterogeneity inside the material. The research results verify the feasibility of applying sand powder 3D printing technology to study the size effect of fractured rock masses and provide an innovative test method for the size effect test study. Preliminary exploration of the size effect of fractured rock masses provides a powerful reference for related research in this field. The study proves the feasibility of applying sand powder 3D printing technology in similar rock mechanics tests and contributes to understanding the size effect of a fractured rock mass.