Pesticide residues have long been a significant aspect of food safety, which has always been a major social concern. This study presents research and analysis on the identification of pesticide residue fast detection cards based on the enzyme inhibition approach. In this study, image recognition technology is used to extract the color information RGB eigenvalues from the detection results of the quick detection card, and four regression models are established to quantitatively predict the pesticide residue concentration indicated by the quick detection card using RGB eigenvalues. The four regression models are linear regression model, quadratic polynomial regression model, exponential regression model and RBF neural network model. Through study and comparison, it has been shown that the exponential regression model is superior at predicting the pesticide residue concentration indicated by the rapid detection card. The correlation value is 0.900, and the root mean square error is 0.106. There will be no negative prediction value when the expected concentration is near to 0. This gives a novel concept and data support for the development of image recognition equipment for pesticide residue fast detection cards based on the enzyme inhibition approach.