This study investigated the use of ozone in a rotating packed bed (RPB) with liquid detention for the treatment of Basic Red 46 (BR-46). Liquid detention means that liquid accumulates at the lower section to a certain level in the RPB, which leads to longer liquid residence time and greater liquid holdup in the packing and cavity in the RPB. The experimental results showed that the presence of liquid detention in the RPB significantly enhanced the BR-46 treatment effect and ozone absorption rate. With 200 mL of liquid detention in the RPB, the decolorization rate, COD degradation rate, and ozone absorption rate were 34.7%, 62.8%, and 80.0% higher than those without liquid detention. The effects of the rotational speed of the RPB, ozone concentration, initial BR-46 concentration, liquid and gas flow rates on BR-46 degradation were also investigated, and it was found that the high-gravity environment is beneficial to the degradation of BR-46. These results suggest that with the utilization of the liquid detention phenomena in the high-gravity devices, the applications of the high-gravity technology can be extended to the processes where a long liquid residence time is required.