In this work, the problematic identification of the main sources of noise occurring from the exploitation of railway vehicles moving at a speed of 200 km/h were analyzed. Within the conducted experimental research, the testing fields were appointed, measurement apparatus selected, and a methodology for conducting measurements was defined, including the assessment of noise on a curve and straight track for electric multiple units of the so-called Pendolino, an Alstom type ETR610 series ED25 train. The measurements were made using a microphone camera Bionic S-112 at a distance of 22 m from the track axis. As a result of the conducted experimental research, it was indicated that the noise resulting from vibrations arising at the wheel-rail contact (rolling noise) was the dominant source of sound.