Abstract
The interest of supplying lignocellulosic materials for producing fermentable sugars has recently emerged in order to diminish the negative environmental effects of fossil fuels. In this study, the Ulmus minor clone Ademuz, characterized for its tolerance to Dutch elm disease and its rapid growth, was evaluated as a source of fermentable sugars. For that, different pretreatments, comprising autohydrolysis, dilute acid hydrolysis, acid catalyzed organosolv, and alkaline extraction, were evaluated at two levels of severity (pretreatment temperatures at 160 °C and 180 °C, except for alkaline extraction at 80 °C and 160 °C); and the resulting pretreated materials were enzymatically hydrolyzed for fermentable sugars production. The major extraction of lignin and hemicellulose was achieved during organosolv (48.9%, lignin; 77.9%, hemicellulose) and acid hydrolysis (39.2%, lignin; 95.0%, hemicellulose) at 180 °C, resulting in the major enzymatic digestibility (67.7%, organosolv; 53.5% acid hydrolysis). Contrarily, under the most favorable conditions for autohydrolysis (180 °C) and alkaline extraction (160 °C), lower extraction of lignin and hemicellulose was produced (4.8%, lignin; 67.2%, hemicellulose, autohydrolysis; 22.6%, lignin; 33.1%, hemicellulose, alkaline extraction), leading to lower enzymatic digestibility (32.1%, autohydrolysis; 39.2%, alkaline extraction). Taking into account the sugars produced during enzymatic hydrolysis of pretreated materials and the solubilized sugars from pretreatment liquors, the highest sugars (glucose and xylose) yield production (28.1%) per gram of biomass from U. minor clone Ademuz was achieved with acid catalyzed organosolv at 180 °C.