Abstract
Purple Alfalfa is an inexpensive, abundant, readily available lignocellulosic material. This work was attempted to develop an efficient combination pretreatment by sequential HClO4−ethyl glycol−H2O (1.2:88.8:10, w/w/w) extraction at 130 °C in 0.5 h and urea/NaOH (urea 12 wt%, NaOH 7 wt%) soaking at −20 °C for 0.5 h for the pretreatment of purple alfalfa. The porosity, morphology, and crystallinity of pretreated purple alfalfa were characterized with SEM, FM, XRD, and FTIR. This combination pretreatment had a significant influence on hemicellulose removal and delignification. The above changes could enhance cellulose accessibility to enzymes and improve the enzymatic digestibility of cellulose. High yields of reducing sugars from pretreated purple alfalfa were obtained at 93.4%. In summary, this combination pretreatment has high potential application in the future.