LAPSE:2023.24700v1
Published Article
LAPSE:2023.24700v1
Preparation and Characterization of Two Different Liposomal Formulations with Bioactive Natural Extract for Multiple Applications
March 28, 2023
Abstract
Liposomes continue to attract great interest due to their increased bioavailability in the body and because the substances encapsulated are protected while maintaining their effectiveness. The aim of this study is to obtain “giant” liposomes by lipid film hydration using a preparation formula with two different phospholipids, phosphatidylcholine (PC) and phosphatidylserine (PS). Firstly, the macro- and microscopic characterization, total phenols content and antioxidant capacity of the plant Stellaria media (L.) Vill. were assessed. Then, Stellaria media (L.) Vill. extract was encapsulated in both formulations (PCE and PSE) and the liposomes were characterized according to their morphology, size distribution and Zeta potential using optical microscopy and dynamic light scattering. The encapsulation efficiency (EE%) was determined using the Folin−Ciocalteu method and the values of both formulations were compared. PC and PCE liposomes with a diameter between 712 and 1000 nm and PS and PSE liposomes with a diameter between 58 and 1000 nm were obtained. The values EE% of Stellaria media (L.) Vill. extract for PCE and PSE were 92.09% and 84.25%, respectively.
Liposomes continue to attract great interest due to their increased bioavailability in the body and because the substances encapsulated are protected while maintaining their effectiveness. The aim of this study is to obtain “giant” liposomes by lipid film hydration using a preparation formula with two different phospholipids, phosphatidylcholine (PC) and phosphatidylserine (PS). Firstly, the macro- and microscopic characterization, total phenols content and antioxidant capacity of the plant Stellaria media (L.) Vill. were assessed. Then, Stellaria media (L.) Vill. extract was encapsulated in both formulations (PCE and PSE) and the liposomes were characterized according to their morphology, size distribution and Zeta potential using optical microscopy and dynamic light scattering. The encapsulation efficiency (EE%) was determined using the Folin−Ciocalteu method and the values of both formulations were compared. PC and PCE liposomes with a diameter between 712 and 1000 nm and PS and PSE liposomes with a diameter between 58 and 1000 nm were obtained. The values EE% of Stellaria media (L.) Vill. extract for PCE and PSE were 92.09% and 84.25%, respectively.
Record ID
Keywords
encapsulation efficiency, film hydration method, liposomes, phospholipids, Stellaria media (L.) Vill., Zeta potential
Subject
Suggested Citation
Miere (Groza) F, Vicas SI, Timar AV, Ganea M, Zdrinca M, Cavalu S, Fritea L, Vicas L, Muresan M, Pallag A, Dobjanschi L. Preparation and Characterization of Two Different Liposomal Formulations with Bioactive Natural Extract for Multiple Applications. (2023). LAPSE:2023.24700v1
Author Affiliations
Miere (Groza) F: Doctoral School of Biomedical Science, University of Oradea, 1 University Street, 410087 Oradea, Romania
Vicas SI: Faculty of Environmental Protection, University of Oradea, 26 Gen. Magheru Street, 410048 Oradea, Romania
Timar AV: Faculty of Environmental Protection, University of Oradea, 26 Gen. Magheru Street, 410048 Oradea, Romania
Ganea M: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Zdrinca M: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Cavalu S: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Fritea L: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Vicas L: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania [ORCID]
Muresan M: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Pallag A: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania [ORCID]
Dobjanschi L: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Vicas SI: Faculty of Environmental Protection, University of Oradea, 26 Gen. Magheru Street, 410048 Oradea, Romania
Timar AV: Faculty of Environmental Protection, University of Oradea, 26 Gen. Magheru Street, 410048 Oradea, Romania
Ganea M: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Zdrinca M: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Cavalu S: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Fritea L: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Vicas L: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania [ORCID]
Muresan M: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Pallag A: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania [ORCID]
Dobjanschi L: Faculty of Medicine and Pharmacy, University of Oradea, 1 Decembrie Street, 410073 Oradea, Romania
Journal Name
Processes
Volume
9
Issue
3
First Page
432
Year
2021
Publication Date
2021-02-27
ISSN
2227-9717
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Original Submission
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PII: pr9030432, Publication Type: Journal Article
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LAPSE:2023.24700v1
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https://doi.org/10.3390/pr9030432
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Mar 28, 2023
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