This study systematically established the most effective refining process for Moringa oleifera (MO) kernel oil. Acid degumming (20.33 ± 1.37 ppm) removed significantly greater phosphorus than water degumming (31.18 ± 0.90 ppm). Neutralization was more effective than deodorization in decreasing the acid (0.06 mg KOH/g) and p-Anisidine (p-AV, 0.36 ± 0.03) values of the oil. Besides improving its color properties, acid-activated bleaching earth Type B was better than Types A and C in decreasing the oil’s p-AV (0.43 ± 0.02), acid value (3.96 ± 0.02 mg KOH/g), and moisture content (0.01 ± 0.00% w/w). The selected refining stages successfully produced MO kernel oil with acceptable peroxide value (PV, 1.66−3.33 meq/kg), p-AV (1.05−1.49), total oxidation value (TOTOX, 4.38−8.15), acid value (0.03 mg KOH/g), moisture content (0.01% w/w), phosphorus content (1.28−1.94 ppm), iodine value (80.79−81.03), oleic acid (79.52−79.65%), and tocopherol content (65.26−87.00 mg/kg).