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LAPSE:2023.2095

Purification and Characterization of a Novel Factor of Crotoxin Inter-CRO (V-1), a New Phospholipase A2 Isoform from Crotalus durissus collilineatus Snake Venom Using an In Vitro Neuromuscular Preparation

Corina Vera-Gonzales, Carlos Alberto Arenas-Chávez, Luis A. Ponce-Soto, Aldo Alvarez-Risco, Shyla Del-Aguila-Arcentales, Neal M. Davies, Jaime A. Yáñez

February 21, 2023

Abstract
The fractionation of Crotalus durissus collilineatus whole venom through an HPLC chromatographic method enabled the purification of a new V-1 neurotoxin. Inter-CRO (V-1) presents similarity in its primary structure to crotoxin B (CB), suggesting another isoform of this toxin. The aim of this study was to compare V-1 to the crotoxin complex (CA/CB) and CB to elucidate aspects related to its functionality. The homogeneity of the purified protein was confirmed with a molecular mass of 1425.45 Da, further verified by mass spectrometry. The sequence of the protein showed high similarity to other viperid snake venom PLA2 proteins. The results of this study report that V-1 is an uncharacterized novel toxin with different biological activities from CB. V-1 maintained catalytic activity but presented neurotoxic activity as observed by the 2.5-fold increase in twitch tension record compared to control values on isolated muscle cells.

Record ID

LAPSE:2023.2095

Keywords

Crotalus durissus collilineatus, Crotalus durissus terrificus, HPLC-RP, neurotoxin, V-1

Subject

Materials

Suggested Citation

Vera-Gonzales C, Arenas-Chávez CA, Ponce-Soto LA, Alvarez-Risco A, Del-Aguila-Arcentales S, Davies NM, Yáñez JA. Purification and Characterization of a Novel Factor of Crotoxin Inter-CRO (V-1), a New Phospholipase A2 Isoform from Crotalus durissus collilineatus Snake Venom Using an In Vitro Neuromuscular Preparation. (2023). LAPSE:2023.2095

Author Affiliations

Vera-Gonzales C: Departamento Académico de Química, Universidad Nacional de San Agustín de Arequipa, Arequipa 04000, Peru
Arenas-Chávez CA: Departamento Académico de Biología, Universidad Nacional de San Agustín de Arequipa, Arequipa 04000, Peru
Ponce-Soto LA: Instituto de Biología, Universidade Estadual de Campinas (UNICAMP), Campinas 13083-970, Brazil; Escuela de Posgrado, Universidad Católica de Santa María, Arequipa 04000, Peru [ORCID]
Alvarez-Risco A: Carrera de Negocios Internacionales, Facultad de Ciencias Empresariales y Económicas, Universidad de Lima, Lima 15023, Peru [ORCID]
Del-Aguila-Arcentales S: Escuela de Posgrado, Universidad San Ignacio de Loyola, Lima 15024, Peru [ORCID]
Davies NM: Faculty of Pharmacy & Pharmaceutical Sciences, University of Alberta, Edmonton, AB T6G 2H1, Canada [ORCID]
Yáñez JA: Vicerrectorado de Investigación, Universidad Norbert Wiener, Lima 15046, Peru; Gerencia Corporativa de Asuntos Científicos y Regulatorios, Teoma Global, Lima 15073, Peru [ORCID]

Journal Name

Processes

Volume

Issue

First Page

1428

Year

2022

Publication Date

2022-07-21

DOI:

https://doi.org/10.3390/pr10071428