Abstract
The aim of this research is to examine the effect of lignocellulosic biomass detoxification on the efficiency of the methane fermentation process. Both for corn straw and rye straw, the methane yield was expressed per volume of fermentation medium and per mass of volatile solids (VS) added. Lignocellulosic biomass was subjected of thermo-chemical and enzymatic sequential pretreatments. It was found that methane yield was higher by 22% when using the detoxification process. In these variants, CH4 yield was 18.86 L/L for corn straw and 17.69 L/L for rye straw; while methane yield expressed per mass of VS added was 0.31 m3/kg VS for corn straw and 0.29 m3/kg VS for rye straw. The inclusion of a detoxification step in pretreatments of biomass lignocellulosic increases the degree of organic substance decomposition and enhances methane yield. The results show that a two-step pretreatment, alkaline/enzymatic with a detoxification process, is necessary for the effective generation of high methane concentration biogas.