LAPSE:2023.1926
Published Article
LAPSE:2023.1926
Vaccine Commercialization: Physiochemical Factors for Optimum Production
February 21, 2023
Abstract
spp. are Gram-negative facultative bacteria that cause severe economic and animal losses. Pasteurella-based vaccines are the most promising solution for controlling Pasteurella spp. outbreaks. Remarkably, insufficient biomass cultivation (low cell viability and productivity) and lack of knowledge about the cultivation process have impacted the bulk production of animal vaccines. Bioprocess optimization in the shake flask and bioreactor is required to improve process efficiency while lowering production costs. However, its state of the art is limited in providing insights on its biomass upscaling, preventing a cost-effective vaccine with mass-produced bacteria from being developed. In general, in the optimum cultivation of Pasteurella spp., production factors such as pH (6.0−8.2), agitation speed (90−500 rpm), and temperature (35−40 °C) are used to improve production yield. Hence, this review discusses the production strategy of Pasteurella and Mannheimia species that can potentially be used in the vaccines for controlling pasteurellosis. The physicochemical factors related to operational parameter process conditions from a bioprocess engineering perspective that maximize yields with minimized production cost are also covered, with the expectation of facilitating the commercialization process.
spp. are Gram-negative facultative bacteria that cause severe economic and animal losses. Pasteurella-based vaccines are the most promising solution for controlling Pasteurella spp. outbreaks. Remarkably, insufficient biomass cultivation (low cell viability and productivity) and lack of knowledge about the cultivation process have impacted the bulk production of animal vaccines. Bioprocess optimization in the shake flask and bioreactor is required to improve process efficiency while lowering production costs. However, its state of the art is limited in providing insights on its biomass upscaling, preventing a cost-effective vaccine with mass-produced bacteria from being developed. In general, in the optimum cultivation of Pasteurella spp., production factors such as pH (6.0−8.2), agitation speed (90−500 rpm), and temperature (35−40 °C) are used to improve production yield. Hence, this review discusses the production strategy of Pasteurella and Mannheimia species that can potentially be used in the vaccines for controlling pasteurellosis. The physicochemical factors related to operational parameter process conditions from a bioprocess engineering perspective that maximize yields with minimized production cost are also covered, with the expectation of facilitating the commercialization process.
Record ID
Keywords
cultivation process, Mannheimia haemolytica, maximizing yields, minimizing cost, Pasteurella multocida, pasteurellosis, physiochemical factors
Subject
Suggested Citation
Oslan SNH, Tan JS, Yusoff AH, Sulaiman AZ, Awang MA, Lazim AM, Lim SJ, Oslan SN, Saad MZ, Ariff AB. Vaccine Commercialization: Physiochemical Factors for Optimum Production. (2023). LAPSE:2023.1926
Author Affiliations
Oslan SNH: Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia; Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, [ORCID]
Tan JS: Bioprocess Technology, School of Industrial Technology, Universiti Sains Malaysia, Gelugor 11800, Pulau Pinang, Malaysia
Yusoff AH: Gold Rare Earth and Material Technopreneurship Centre (GREAT), Faculty of Bioengineering and Technology (FBET), Universiti Malaysia Kelantan Kampus Jeli, Locked Bag 100, Jeli 17600, Kelantan, Malaysia
Sulaiman AZ: Gold Rare Earth and Material Technopreneurship Centre (GREAT), Faculty of Bioengineering and Technology (FBET), Universiti Malaysia Kelantan Kampus Jeli, Locked Bag 100, Jeli 17600, Kelantan, Malaysia [ORCID]
Awang MA: Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia
Lazim AM: Department of Chemical, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia
Lim SJ: Enzyme and Microbial Technology Research Centre, Centre of Excellence, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia; Enzyme Technology and X-ray Crystallography Laboratory, VacBio 5, Institute of Bioscience, Universiti Putra Malaysia, Serd [ORCID]
Oslan SN: Enzyme and Microbial Technology Research Centre, Centre of Excellence, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia; Enzyme Technology and X-ray Crystallography Laboratory, VacBio 5, Institute of Bioscience, Universiti Putra Malaysia, Serd [ORCID]
Saad MZ: Research Centre for Ruminant Diseases, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia [ORCID]
Ariff AB: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
Tan JS: Bioprocess Technology, School of Industrial Technology, Universiti Sains Malaysia, Gelugor 11800, Pulau Pinang, Malaysia
Yusoff AH: Gold Rare Earth and Material Technopreneurship Centre (GREAT), Faculty of Bioengineering and Technology (FBET), Universiti Malaysia Kelantan Kampus Jeli, Locked Bag 100, Jeli 17600, Kelantan, Malaysia
Sulaiman AZ: Gold Rare Earth and Material Technopreneurship Centre (GREAT), Faculty of Bioengineering and Technology (FBET), Universiti Malaysia Kelantan Kampus Jeli, Locked Bag 100, Jeli 17600, Kelantan, Malaysia [ORCID]
Awang MA: Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia
Lazim AM: Department of Chemical, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Selangor, Malaysia
Lim SJ: Enzyme and Microbial Technology Research Centre, Centre of Excellence, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia; Enzyme Technology and X-ray Crystallography Laboratory, VacBio 5, Institute of Bioscience, Universiti Putra Malaysia, Serd [ORCID]
Oslan SN: Enzyme and Microbial Technology Research Centre, Centre of Excellence, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia; Enzyme Technology and X-ray Crystallography Laboratory, VacBio 5, Institute of Bioscience, Universiti Putra Malaysia, Serd [ORCID]
Saad MZ: Research Centre for Ruminant Diseases, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia [ORCID]
Ariff AB: Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
Journal Name
Processes
Volume
10
Issue
7
First Page
1248
Year
2022
Publication Date
2022-06-23
ISSN
2227-9717
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PII: pr10071248, Publication Type: Review
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LAPSE:2023.1926
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https://doi.org/10.3390/pr10071248
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