LAPSE:2023.1403
Published Article
LAPSE:2023.1403
Experimental Characterization of a Microfluidic Device Based on Passive Crossflow Filters for Blood Fractionation
February 21, 2023
Abstract
The separation of red blood cells (RBCs) from blood plasma and the analysis of individual RBCs are of great importance, as they provide valuable information regarding the health of their donor. Recent developments in microfluidics and microfabrication have contributed to the fabrication of microsystems with complex features to promote the separation and analysis of RBCs. In this work, the separation capacity of a multi-step crossflow microfluidic device was evaluated by using a blood analogue fluid made by Brij L4 micelles and human RBCs separated from whole blood, suspended in a solution with hematocrits (Ht) of 0.5 and 1%. All the samples collected at the outlets of the device were experimentally analyzed and compared. The absorbance spectrum was also measured for the prepared blood samples. The results indicate that the tested blood analogue fluid has exhibited a flow behavior similar to that of blood. In addition, the optical absorbance spectrophotometry revealed that it was possible to evaluate the separation efficiency of the microfluidic device, concluding that the concentration of cells was lower at the most lateral outside outlets of the microchannel due to the cumulative effect of the multiple cross-flow filters.
The separation of red blood cells (RBCs) from blood plasma and the analysis of individual RBCs are of great importance, as they provide valuable information regarding the health of their donor. Recent developments in microfluidics and microfabrication have contributed to the fabrication of microsystems with complex features to promote the separation and analysis of RBCs. In this work, the separation capacity of a multi-step crossflow microfluidic device was evaluated by using a blood analogue fluid made by Brij L4 micelles and human RBCs separated from whole blood, suspended in a solution with hematocrits (Ht) of 0.5 and 1%. All the samples collected at the outlets of the device were experimentally analyzed and compared. The absorbance spectrum was also measured for the prepared blood samples. The results indicate that the tested blood analogue fluid has exhibited a flow behavior similar to that of blood. In addition, the optical absorbance spectrophotometry revealed that it was possible to evaluate the separation efficiency of the microfluidic device, concluding that the concentration of cells was lower at the most lateral outside outlets of the microchannel due to the cumulative effect of the multiple cross-flow filters.
Record ID
Keywords
blood analogue, blood on chip, cell separation, crossflow microfluidic device, optical absorbance spectrophotometry, passive microfluidic device
Subject
Suggested Citation
Gonçalves IM, Castro I, Barbosa F, Faustino V, Catarino SO, Moita A, Miranda JM, Minas G, Sousa PC, Lima R. Experimental Characterization of a Microfluidic Device Based on Passive Crossflow Filters for Blood Fractionation. (2023). LAPSE:2023.1403
Author Affiliations
Gonçalves IM: Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimarães, Portugal; IN+, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal [ORCID]
Castro I: Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimarães, Portugal
Barbosa F: Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimarães, Portugal [ORCID]
Faustino V: Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimarães, Portugal; Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, 4800-058 Guimarães, Portugal; LABBELS—Associate Labo [ORCID]
Catarino SO: Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, 4800-058 Guimarães, Portugal; LABBELS—Associate Laboratory, 4710-057 Braga, Portugal [ORCID]
Moita A: IN+, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal; Centro de Investigação Desenvolvimento e Inovação da Academia Militar, Academia Militar, Instituto Universitário Militar, 1169-203 Lisboa, Portugal [ORCID]
Miranda JM: CEFT—Transport Phenomena Research Center, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal; ALiCE—Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal [ORCID]
Minas G: Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, 4800-058 Guimarães, Portugal; LABBELS—Associate Laboratory, 4710-057 Braga, Portugal [ORCID]
Sousa PC: INL—International Iberian Nanotechnology Laboratory, 4715-330 Braga, Portugal [ORCID]
Lima R: Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimarães, Portugal; CEFT—Transport Phenomena Research Center, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal; ALiCE—Associate L [ORCID]
Castro I: Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimarães, Portugal
Barbosa F: Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimarães, Portugal [ORCID]
Faustino V: Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimarães, Portugal; Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, 4800-058 Guimarães, Portugal; LABBELS—Associate Labo [ORCID]
Catarino SO: Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, 4800-058 Guimarães, Portugal; LABBELS—Associate Laboratory, 4710-057 Braga, Portugal [ORCID]
Moita A: IN+, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal; Centro de Investigação Desenvolvimento e Inovação da Academia Militar, Academia Militar, Instituto Universitário Militar, 1169-203 Lisboa, Portugal [ORCID]
Miranda JM: CEFT—Transport Phenomena Research Center, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal; ALiCE—Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal [ORCID]
Minas G: Center for MicroElectromechanical Systems (CMEMS-UMinho), University of Minho, 4800-058 Guimarães, Portugal; LABBELS—Associate Laboratory, 4710-057 Braga, Portugal [ORCID]
Sousa PC: INL—International Iberian Nanotechnology Laboratory, 4715-330 Braga, Portugal [ORCID]
Lima R: Mechanical Engineering and Resource Sustainability Center (METRICS), University of Minho, 4800-058 Guimarães, Portugal; CEFT—Transport Phenomena Research Center, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal; ALiCE—Associate L [ORCID]
Journal Name
Processes
Volume
10
Issue
12
First Page
2698
Year
2022
Publication Date
2022-12-14
ISSN
2227-9717
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PII: pr10122698, Publication Type: Journal Article
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LAPSE:2023.1403
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https://doi.org/10.3390/pr10122698
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