LAPSE:2020.1264
Published Article
LAPSE:2020.1264
Tetracycline Induces the Formation of Biofilm of Bacteria from Different Phases of Wastewater Treatment
December 22, 2020
The study monitored the effect of tetracycline on bacterial biofilm formation and compared biofilm formation by resistant bacterial strains in different phases of the wastewater treatment process in wastewater treatment plant (WWTP). The crystal violet staining method was used to evaluate the biofilm formation. Biofilm-related bacterial properties were characterized by hydrophobicity, autoaggregation and motility tests. The relative abundance of tetracycline resistance genes (tetW, tetM, tetO, tetA and tetB) in wastewaters were subsequently quantified using qPCR. The results show that the isolates from the nitrification tank produce biofilm with up to 10 times greater intensity relative to the isolates from the sedimentation tank. In isolates of Aeromonas sp. from the nitrification tank, increased biofilm production in the occurrence of tetracycline from a concentration of 0.03125 µg/mL was observed. The tetW gene showed the highest relative abundance out of all the tested genes. From the sampling points, its abundance was the highest in the sedimentation tank of the WWTP. Based on these results, it can be assumed that resistant bacteria are able to form a biofilm and sub-inhibitory tetracycline concentrations induce biofilm formation. WWTPs thus represent a reservoir of antibiotic resistance genes and contribute to the spread of resistance in the natural environment.
Keywords
biofilm, tetracycline, tetracycline resistance bacteria, tetracycline resistance genes, wastewater treatment plant
Subject
Suggested Citation
Stachurová T, Malachová K, Semerád J, Sterniša M, Rybková Z, Smole Možina S. Tetracycline Induces the Formation of Biofilm of Bacteria from Different Phases of Wastewater Treatment. (2020). LAPSE:2020.1264
Author Affiliations
Stachurová T: Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, CZ-710 00 Ostrava, Czech Republic [ORCID]
Malachová K: Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, CZ-710 00 Ostrava, Czech Republic [ORCID]
Semerád J: Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, CZ-142 20 Prague 4, Czech Republic; Institute for Environmental Studies, Faculty of Science, Charles University, Benátská 2, CZ-128 01 Prague 2, Czech Republic [ORCID]
Sterniša M: Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia [ORCID]
Rybková Z: Department of Biology and Ecology, Faculty of Science, University of Ostrava, Chittussiho 10, CZ-710 00 Ostrava, Czech Republic
Smole Možina S: Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia [ORCID]
Journal Name
Processes
Volume
8
Issue
8
Article Number
E989
Year
2020
Publication Date
2020-08-14
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr8080989, Publication Type: Journal Article
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LAPSE:2020.1264
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doi:10.3390/pr8080989
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Dec 22, 2020
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CC BY 4.0
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Dec 22, 2020
 
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Calvin Tsay
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