Whether the variation of pore structures and movable fluid characteristics enhance, deteriorate, or have no influence on reservoir quality has long been disputed, despite their considerable implications for hydrocarbon development in tight sandstone reservoirs. To elucidate these relationships, this study systematically analyzes pore structures qualitatively and quantitatively by various kinds of direct observations, indirect methods, and imaging simulations. We found that the uncertainty of porosity measurements, caused by the complex pore-throat structure, needs to be eliminated to accurately characterize reservoir quality. Bulk water was more easily removed, while surface water tended to be retained in the pores, and the heterogeneity of pore structures was caused by the abundance of tiny pores. The rates of water saturation reduction in macropores are faster than those for tiny pores, and sandstones with poor reservoir quality show no marked descending of lower limits of movable pore radius, indicating that the movable fluid would advance exempted from the larger pores. This study suggests that the deterioration of reservoir quality is strongly affected by the reduction of larger pores and the aqueous phases tended to remain in the tiny pores in the forms of surface water.