LAPSE:2020.0909
Published Article
LAPSE:2020.0909
A Reliable Automated Sampling System for On-Line and Real-Time Monitoring of CHO Cultures
August 5, 2020
Timely monitoring and control of critical process parameters and product attributes are still the basic tasks in bioprocess development. The current trend of automation and digitization in bioprocess technology targets an improvement of these tasks by reducing human error and increasing through-put. The gaps in such automation procedures are still the sampling procedure, sample preparation, sample transfer to analyzers, and the alignment of process and sample data. In this study, an automated sampling system and the respective data management software were evaluated for system performance; applicability with HPLC for measurement of vitamins, product and amino acids; and applicability with a biochemical analyzer. The focus was especially directed towards the adaptation and assessment of an appropriate amino acid method, as these substances are critical in cell culture processes. Application of automated sampling in a CHO fed-batch revealed its potential with regard to data evaluation. The higher sampling frequency compared to manual sampling increases the generated information content, which allows easier interpretation of the metabolism, extraction of e.g., ks values, application of smoothing algorithms, and more accurate detection of process events. A comparison with sensor technology shows the advantages and disadvantages in terms of measurement errors and measurement frequency.
Record ID
Keywords
amino acids, automated sampling, bioprocess, CHO, process analytical technology, vitamins
Subject
Suggested Citation
Hofer A, Kroll P, Barmettler M, Herwig C. A Reliable Automated Sampling System for On-Line and Real-Time Monitoring of CHO Cultures. (2020). LAPSE:2020.0909
Author Affiliations
Hofer A: Research Area Biochemical Engineering, Institute of Chemical, Environmental and Biological Engineering, Vienna University of Technology, Gumpendorferstrasse 1a-166/4, 1060 Vienna, Austria
Kroll P: Research Area Biochemical Engineering, Institute of Chemical, Environmental and Biological Engineering, Vienna University of Technology, Gumpendorferstrasse 1a-166/4, 1060 Vienna, Austria; CD Laboratory on Mechanistic and Physiological Methods for Improve
Barmettler M: Securecell AG, In der Luberzen 29, 8902 Urdorf, Switzerland
Herwig C: Research Area Biochemical Engineering, Institute of Chemical, Environmental and Biological Engineering, Vienna University of Technology, Gumpendorferstrasse 1a-166/4, 1060 Vienna, Austria; CD Laboratory on Mechanistic and Physiological Methods for Improve [ORCID]
Kroll P: Research Area Biochemical Engineering, Institute of Chemical, Environmental and Biological Engineering, Vienna University of Technology, Gumpendorferstrasse 1a-166/4, 1060 Vienna, Austria; CD Laboratory on Mechanistic and Physiological Methods for Improve
Barmettler M: Securecell AG, In der Luberzen 29, 8902 Urdorf, Switzerland
Herwig C: Research Area Biochemical Engineering, Institute of Chemical, Environmental and Biological Engineering, Vienna University of Technology, Gumpendorferstrasse 1a-166/4, 1060 Vienna, Austria; CD Laboratory on Mechanistic and Physiological Methods for Improve [ORCID]
Journal Name
Processes
Volume
8
Issue
6
Article Number
E637
Year
2020
Publication Date
2020-05-27
Published Version
ISSN
2227-9717
Version Comments
Original Submission
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PII: pr8060637, Publication Type: Journal Article
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Published Article
LAPSE:2020.0909
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External Link
doi:10.3390/pr8060637
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Version History
[v1] (Original Submission)
Aug 5, 2020
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Aug 5, 2020
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https://psecommunity.org/LAPSE:2020.0909
Original Submitter
Calvin Tsay
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