LAPSE:2020.0485
Published Article
LAPSE:2020.0485
Optimizing the Extraction of Polysaccharides from Bletilla ochracea Schltr. Using Response Surface Methodology (RSM) and Evaluating their Antioxidant Activity
May 22, 2020
Bletilla ochracea Schltr. polysaccharides (BOP) have a similar structure to Bletilla striata (Thunb.) Reichb.f. (Orchidaceae) polysaccharides (BSP). Therefore, BOP can be considered as a substitute for BSP in the food, pharmaceuticals and cosmetics fields. To the best of our knowledge, little information is available regarding the optimization of extraction and antioxidant activity of BOP. In this study, response surface methodology (RSM) was firstly used for optimizing the extraction parameters of BOP. The results suggested that the optimal conditions included a temperature of 82 °C, a duration of 85 min and a liquid/material ratio of 30 mL/g. In these conditions, we received 26.45% ± 0.18% as the experimental yield. In addition, BOP exhibited strong concentration-dependent antioxidant abilities in vitro. The half-maximal effective concentration (EC50) values of BOP against 1,1-diphenyl-2-picrylhydrazyl (DPPH·), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonate) (ABTS+·), hydroxyl (·OH) and superoxide anion (·O2−) radicals and ferrous ions (Fe2+) were determined as 692.16, 224.09, 542.22, 600.53 and 515.70 µg/mL, respectively. In conclusion, our results indicate that BOP can be a potential natural antioxidant, deserving further investigation.
Record ID
Keywords
antioxidant activities, Bletilla ochracea Schltr. polysaccharide, response surface methodology (RSM)
Subject
Suggested Citation
Wang B, Xu Y, Chen L, Zhao G, Mi Z, Lv D, Niu J. Optimizing the Extraction of Polysaccharides from Bletilla ochracea Schltr. Using Response Surface Methodology (RSM) and Evaluating their Antioxidant Activity. (2020). LAPSE:2020.0485
Author Affiliations
Wang B: National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal Univ
Xu Y: National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal Univ
Chen L: National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal Univ
Zhao G: National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal Univ
Mi Z: National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal Univ
Lv D: Shanxi Institute of Medicine and Life Sciences, Taiyuan 030006, China
Niu J: National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal Univ
Xu Y: National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal Univ
Chen L: National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal Univ
Zhao G: National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal Univ
Mi Z: National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal Univ
Lv D: Shanxi Institute of Medicine and Life Sciences, Taiyuan 030006, China
Niu J: National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal Univ
Journal Name
Processes
Volume
8
Issue
3
Article Number
E341
Year
2020
Publication Date
2020-03-16
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr8030341, Publication Type: Journal Article
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LAPSE:2020.0485
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doi:10.3390/pr8030341
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[v1] (Original Submission)
May 22, 2020
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May 22, 2020
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https://psecommunity.org/LAPSE:2020.0485
Original Submitter
Calvin Tsay
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