Size-Selected Graphene Oxide Loaded with Photosensitizer (TMPyP) for Targeting Photodynamic Therapy In Vitro

Kateřina Bartoň Tománková; Ariana Opletalová; Kateřina Poláková; Sergii Kalytchuk; Jana Jiravová; Jakub Malohlava; Lukáš Malina; Hana Kolářová

LAPSE

Living Archive for Process Systems Engineering

LAPSE:2020.0396

Published Article

LAPSE:2020.0396

Size-Selected Graphene Oxide Loaded with Photosensitizer (TMPyP) for Targeting Photodynamic Therapy In Vitro

Kateřina Bartoň Tománková, Ariana Opletalová, Kateřina Poláková, Sergii Kalytchuk, Jana Jiravová, Jakub Malohlava, Lukáš Malina, Hana Kolářová

April 14, 2020

Targeted therapies of various diseases are nowadays widely studied in many biomedical fields. Photodynamic therapy (PDT) represents a modern treatment of cancer using a locally activated light. TMPyP is an efficient synthetic water-soluble photosensitizer (PS), yet with poor absorption in the visible and the red regions. In this work, we prepared size-selected and colloidally stable graphene oxide (GO) that is appropriate for biomedical use. Thanks to the negative surface charge of GO, TMPyP was easily linked in order to create conjugates of GO/TMPyP by electrostatic force. Due to the strong ionic interactions, charge transfers between GO and TMPyP occur, as comprehensively investigated by steady-state and time-resolved fluorescence spectroscopy. Biocompatibility and an in vitro effect of GO/TMPyP were confirmed by a battery of in vitro tests including MTT, comet assay, reactive oxygen species (ROS) production, and monitoring the cellular uptake. PDT efficiency of GO/TMPyP was tested using 414 and 740 nm photoexcitation. Our newly prepared nanotherapeutics showed a higher PDT effect than in free TMPyP, and is promising for targeted therapy using clinically favorable conditions.

Record ID

LAPSE:2020.0396

Keywords

in vitro, photodynamic therapy, photosensitizer, size reduced graphene oxide, TMPyP

Subject

Biosystems

Suggested Citation

Bartoň Tománková K, Opletalová A, Poláková K, Kalytchuk S, Jiravová J, Malohlava J, Malina L, Kolářová H. Size-Selected Graphene Oxide Loaded with Photosensitizer (TMPyP) for Targeting Photodynamic Therapy In Vitro. (2020). LAPSE:2020.0396

Author Affiliations

Bartoň Tománková K: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Institute of Translational Medicine, Palacký University Olomouc, Hněvotínská 3, 775 15 Olomouc, Czech Republic [ORCID]
Opletalová A: Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, Šlechtitelů 27, 771 46 Olomouc, Czech Republic
Poláková K: Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, Šlechtitelů 27, 771 46 Olomouc, Czech Republic
Kalytchuk S: Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University, Šlechtitelů 27, 771 46 Olomouc, Czech Republic [ORCID]
Jiravová J: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Institute of Translational Medicine, Palacký University Olomouc, Hněvotínská 3, 775 15 Olomouc, Czech Republic
Malohlava J: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Institute of Translational Medicine, Palacký University Olomouc, Hněvotínská 3, 775 15 Olomouc, Czech Republic [ORCID]
Malina L: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Institute of Translational Medicine, Palacký University Olomouc, Hněvotínská 3, 775 15 Olomouc, Czech Republic
Kolářová H: Department of Medical Biophysics, Faculty of Medicine and Dentistry, Institute of Translational Medicine, Palacký University Olomouc, Hněvotínská 3, 775 15 Olomouc, Czech Republic

Journal Name

Processes

Volume

8

Issue

2

Article Number

E251

Year

2020

Publication Date

2020-02-24