The optimal P-Q control issue of the active and reactive power for a microgrid in the grid-connected mode has attracted increasing interests recently. In this paper, an optimal active and reactive power control is developed for a three-phase grid-connected inverter in a microgrid by using an adaptive population-based extremal optimization algorithm (APEO). Firstly, the optimal P-Q control issue of grid-connected inverters in a microgrid is formulated as a constrained optimization problem, where six parameters of three decoupled PI controllers are real-coded as the decision variables, and the integral time absolute error (ITAE) between the output and referenced active power and the ITAE between the output and referenced reactive power are weighted as the objective function. Then, an effective and efficient APEO algorithm with an adaptive mutation operation is proposed for solving this constrained optimization problem. The simulation and experiments for a 3 kW three-phase grid-connected inverter under both nominal and variable reference active power values have shown that the proposed APEO-based P-Q control method outperforms the traditional Z-N empirical method, the adaptive genetic algorithm-based, and particle swarm optimization-based P-Q control methods.