Threshold pressure gradient (TPG) is a key parameter determining the pore-scale fluid dynamics. In tight gas reservoirs, both gas and water exist in the porous rock, and the existing water can be divided into irreducible and movable water. However, how movable water saturation will influence TPG has not yet been investigated. Therefore herein, nuclear magnetic resonance (NMR) and high-pressure mercury intrusion (HPMI) experiments were performed to determine pore-scale water distribution, movable water saturation, and pore throat distribution in the core plugs. Subsequently, the air bubble method was used to measure TPG as a function of movable water saturation and permeability inside tight gas core plugs, finding that TPG increased from 0.01 MPa/m to 0.25 MPa/m with the movable saturation increased from 2% to 35%. Finally, a semi-empirical model was derived to describe the correlation between TPG, movable water saturation, and permeability, which performed better than previous models in the literature. These insights will advance the fundamental understanding of TPG in tight gas reservoirs and provide useful guidance on tight gas reservoirs development.