LAPSE:2023.26180
Published Article
LAPSE:2023.26180
Saccharification Yield through Enzymatic Hydrolysis of the Steam-Exploded Pinewood
March 31, 2023
Pressure, temperature, and retention time are the most studied parameters in steam explosion pretreatment. However, this work aimed to fix these parameters and to evaluate the influences of several less investigated steam explosion parameters on the saccharification yield in hydrolysis. In this study, firstly, pinewood samples smaller than 200 µm were treated with steam explosion at 190 °C for 10 min. The variable parameters were biomass loading, N2 pressure, and release time. Steam-exploded samples were hydrolyzed with the Trichoderma reesei enzyme for saccharification for 72 h. The sugar content of the resultant products was analyzed to estimate the yield of sugars (such as glucose, xylose, galactose, mannose, and arabinose). The best glucose yield in the pulp was achieved with 4 g of sample, N2 pressure of 0.44 MPa, and short release time (22 s). These conditions gave a glucose yield of 97.72% in the pulp, and the xylose, mannose, galactose, and arabinose yields in the liquid fraction were found to be 85.59%, 87.76%, 86.43%, and 90.3%, respectively.
Keywords
enzymatic hydrolysis, high-performance liquid chromatography (HPLC) analysis, hydrothermal pretreatment, lignocellulosic biomass, sugar yield
Subject
Suggested Citation
Borand MN, Isler Kaya A, Karaosmanoglu F. Saccharification Yield through Enzymatic Hydrolysis of the Steam-Exploded Pinewood. (2023). LAPSE:2023.26180
Author Affiliations
Borand MN: Energy Systems Engineering Department, Faculty of Engineering, Merkez Campus, Yalova University, 77100 Yalova, Turkey [ORCID]
Isler Kaya A: Chemical Engineering Department, Faculty of Chemical and Metallurgical Engineering, Ayazaga Campus Istanbul Technical University, 34467 Istanbul, Turkey
Karaosmanoglu F: Chemical Engineering Department, Faculty of Chemical and Metallurgical Engineering, Ayazaga Campus Istanbul Technical University, 34467 Istanbul, Turkey [ORCID]
Journal Name
Energies
Volume
13
Issue
17
Article Number
E4552
Year
2020
Publication Date
2020-09-02
Published Version
ISSN
1996-1073
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Original Submission
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PII: en13174552, Publication Type: Journal Article
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LAPSE:2023.26180
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doi:10.3390/en13174552
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Mar 31, 2023
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