Many potential uses of carbon nanotubes (CNT) in various sectors have created an urge to assess their diverse range of properties pertaining to various applications like catalysis, biosensor, and antimicrobial activity. Increasing studies on the biosensor and antibacterial activity of CNT have prompted tremendous interest in the utilization of the carbon-based nanostructured material as an alternative to currently existing antibiotics. However, the study of bactericidal aspects of this nanomaterial is relatively new and hence the deeper understanding of the various physicochemical characteristics and antimicrobial nature of CNT is extremely wanted. This review covers the effect of framework substitution and explains the understanding of membrane disintegration and oxidative stresses upon nanomaterials for antimicrobial activity. The present article has also reviewed effect of preparation nanoparticle deposition and framework modification on carbon nanotube structure. The recent research on graphene-modified nanomaterials for biosensor applications related to healthcare/clinical applications have also been discussed. Major physicochemical contributing factors such as size, functionalization, high surface area, and aggregation features of CNT assisting in the bacterial killing have nicely been outlined. Hence, the present review explains the supporting information related with Single and multi-walled carbon nanotube and summarized the advantages of functionalized carbon nanotube/graphene-based nanostructured carbon-based materials towards protection and reduction of bacterial/viral infections in the healthcare sector.