LAPSE:2024.1970
Published Article
LAPSE:2024.1970
Bacteriostatic Activity of Janthinobacterium lividum and Purified Violacein Fraction against Clavibacter michiganensis
August 28, 2024
causes plant diseases and is included in the list of microorganisms subject to export control. Janthinobacterium lividum is capable of synthesizing a pigment with antagonistic potential. The purpose of the study was to evaluate the activity of J. lividum VKM B-3705D and the pigment fraction against C. michiganensis VKM Ac-1402. The results of spectrophotometric and nuclear magnetic resonance analysis showed that the pigment synthesized by the J. lividum VKM B-3705D corresponds to violacein. The J. lividum strain demonstrated potential bacteriostatic activity against C. michiganensis VKM Ac-1402 when both strains were co-cultured. Compared to the control (DMSO), the violacein solution suppressed the specific growth of Clavibacter by 57.7%. The mechanism of suppression of the growth of Clavibacter is discussed. One of the ways to suppress the growth of C. michiganensis may be the inhibition of key enzymes. Violacein inhibited the activity of adenosine triphosphatase (ATPase, EC 3.6.1.3) compared to the control (DMSO) by 23.2%. Thus, the current study of the bacteriostatic effect may be a decisive step towards the development of a plant protection product.
Keywords
adenosine triphosphatase, ATP-ase, bacteriostatic activity, Clavibacter michiganensis, intermicrobial interactions, Janthinobacterium lividum, violacein
Subject
Suggested Citation
Lyakhovchenko NS, Efimova VA, Seliverstov ES, Anis’kov AA, Solyanikova IP. Bacteriostatic Activity of Janthinobacterium lividum and Purified Violacein Fraction against Clavibacter michiganensis. (2024). LAPSE:2024.1970
Author Affiliations
Lyakhovchenko NS: Regional Microbiological Center, Institute of Pharmacy, Chemistry and Biology, Belgorod National Research University, Pobeda Street, 85, Belgorod 308015, Russia [ORCID]
Efimova VA: Departmrnt of Biotechnology and Microbiology, Institute of Pharmacy, Chemistry and Biology, Belgorod National Research University, Pobeda Street, 85, Belgorod 308015, Russia
Seliverstov ES: Departmrnt of Biotechnology and Microbiology, Institute of Pharmacy, Chemistry and Biology, Belgorod National Research University, Pobeda Street, 85, Belgorod 308015, Russia [ORCID]
Anis’kov AA: Department of Chemistry, Institute of Pharmacy, Chemistry and Biology, Belgorod National Research University, Pobeda Street, 85, Belgorod 308015, Russia [ORCID]
Solyanikova IP: Regional Microbiological Center, Institute of Pharmacy, Chemistry and Biology, Belgorod National Research University, Pobeda Street, 85, Belgorod 308015, Russia [ORCID]
Journal Name
Processes
Volume
12
Issue
6
First Page
1116
Year
2024
Publication Date
2024-05-29
ISSN
2227-9717
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Original Submission
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PII: pr12061116, Publication Type: Journal Article
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LAPSE:2024.1970
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https://doi.org/10.3390/pr12061116
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Aug 28, 2024
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