A Promising Biocontrol Agent of Bacillus velezensis VC3 against Magnaporthe oryzae and Colletotrichum gloeosporioides in Plants

Kui Li; Xin Zhang; Yue Chen; Tong Zhang; Qianlong Sun; Hongtao Wu; Zhen Chen; Yan Sun; Dehua Liu; Xinqiu Tan

LAPSE

Living Archive for Process Systems Engineering

LAPSE:2024.1845

Published Article

LAPSE:2024.1845

A Promising Biocontrol Agent of Bacillus velezensis VC3 against Magnaporthe oryzae and Colletotrichum gloeosporioides in Plants

Kui Li, Xin Zhang, Yue Chen, Tong Zhang, Qianlong Sun, Hongtao Wu, Zhen Chen, Yan Sun, Dehua Liu, Xinqiu Tan

August 23, 2024

Fungal diseases of plants are one of the key factors causing global crop losses. In this study, we isolated a Bacillus velezensis strain VC3, which was found to have a broad-spectrum inhibitory effect on a variety of phytopathogenic fungi through in vitro and in planta experiments, especially on Magnaporthe oryzae and Colletotrichum gloeosporioides. Further genomic and transcriptomic analyses revealed that the B. velezensis VC3 has multiple functional gene clusters encoding for the synthesis of a variety of antifungal secondary metabolites, including antimicrobial peptides (AMPs) and lipopeptides (LPs). In addition, AMPs and LPs were isolated and purified from B. velezensis VC3 fermentation broth and their antifungal activities were verified in this study. AMPs and LPs significantly inhibited spore germination, appressorium formation, and disease development, and AMPs have a better potential for controlling M. oryzae and C. gloeosporioides than LPs. These findings open new avenues for utilizing B. velezensis VC3 as biocontrol agents, providing potential sustainable solutions for agricultural production.

Record ID

LAPSE:2024.1845

Keywords

antifungal activity, Bacillus velezensis, biocontrol, phytopathogenic fungi, secondary metabolites

Subject

Biosystems

Suggested Citation

Li K, Zhang X, Chen Y, Zhang T, Sun Q, Wu H, Chen Z, Sun Y, Liu D, Tan X. A Promising Biocontrol Agent of Bacillus velezensis VC3 against Magnaporthe oryzae and Colletotrichum gloeosporioides in Plants. (2024). LAPSE:2024.1845

Author Affiliations

Li K: Key Laboratory of Industrial Biocatalysis (Ministry of Education), Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Zhang X: Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China; Longping Branch, College of Biology, Hunan University, Changsha 410125, China; Yuelushan Laboratory, Changsha 410082, China
Chen Y: Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China; Longping Branch, College of Biology, Hunan University, Changsha 410125, China [ORCID]
Zhang T: Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China
Sun Q: Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China
Wu H: Jiangsu Tsingda Smart Biotech Co., Ltd., Taicang 215400, China
Chen Z: Key Laboratory of Industrial Biocatalysis (Ministry of Education), Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Sun Y: Jiangsu Tsingda Smart Biotech Co., Ltd., Taicang 215400, China
Liu D: Key Laboratory of Industrial Biocatalysis (Ministry of Education), Department of Chemical Engineering, Tsinghua University, Beijing 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China
Tan X: Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China; Longping Branch, College of Biology, Hunan University, Changsha 410125, China; Yuelushan Laboratory, Changsha 410082, China

Journal Name

Processes

Volume

12

Issue

7

First Page

1490

Year

2024

Publication Date

2024-07-16