Abstract
The aim of this study was to optimize parameters of microwave-assisted extraction (MAE) and accelerated solvent extraction (ASE) in terms of extraction temperature and time, microwave power and cycle numbers on the phenolic content of sea buckthorn leaves and berries, using 70% ethanol (v/v) as an extraction solvent. The characterization of phenolic composition in leaf and berry extracts obtained at optimal MAE and ASE conditions was performed with UPLC/ESI-MS2, while antioxidant activity was determined using the ORAC method. The optimal extraction conditions for MAE were 60 °C, 500 W and 15 min for leaves and 60 °C, 300 W, and 10 min for berries. The optimal extraction conditions for ASE from both leaves and berries were 120 °C, 15 min, and 3 cycles. Total phenolic content (TPC) in MAE and ASE extracts from leaves was similar to the TPC determined in extracts obtained by conventional extraction (60 °C/30 min); however, ASE contributed to the higher TPC of the berry extracts. The flavonols kaempferol-3-rutinoside in the leaves and kaempferol in the berries were the most abundant phenols of sea buckthorn. A higher antioxidant activity was found in the leaf extracts obtained by ASE and it correlated with the phenolic content. In general, ASE favored the extraction of all polyphenols from leaves, while MAE was more suitable for the extraction of flavonols from berries, suggesting that the choice of the optimal extraction method is crucial with regard to the target molecules and future applications.