Egypt faces significant challenges in managing its sewage sludge generated in large quantities from wastewater treatment plants. This study investigates the feasibility of utilizing sewage sludge as a renewable resource for hydrogen production through anaerobic digestion at the 100 L bioreactor level. Hydrogen is considered a promising alternative energy source due to its high energy content and environmental benefits. To optimize the microbial degradation process and maximize hydrogen production from sewage sludge, a specialized pretreatment is necessary. Various pretreatment methods have been applied to the sewage sludge, individually and in combination, to study the bio-hydrogen production from sewage sludge. The four methods of treatment were studied in batch assays as a pilot scale. Thermal pretreatment of sewage sludge significantly increases bio-hydrogen production yield compared to other sewage sludge pretreatment methods, producing the highest H2 yield (6.48 LH2/g VS). In general, the hydrogen yield of any type of pretreated inoculum was significantly higher than the untreated inoculum. At the same time, alkaline pretreatment improved the hydrogen yield (1.04 LH2/g VS) more than acid pretreatment (0.74 LH2/g VS), while the hydrogen yield for the combination of pretreatments (shock alkali pretreatment) was higher than both (1.73 LH2/g VS), On the other hand, untreated sewage sludge (control) had almost no hydrogen yield (0.03 LH2/g VS). The self-fermented anaerobic bioreactor improved sewage sludge utilization, increased bioenergy yields, and seems to be promising for treating complex wastes at this scale.