LAPSE:2023.5577
Published Article
LAPSE:2023.5577
Diversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791T
February 23, 2023
is a relatively widely studied, yet non-model, bacterium. While 246 genome assemblies of its various strains are available currently, the diversity of the whole species has not been studied, and it has only been analyzed in part for a missing genome of the type strain. Here, we sequenced and assembled the complete genome of the type strain Clostridium beijerinckii DSM 791T, composed of a circular chromosome and a circular megaplasmid, and used it for a comparison with other genomes to evaluate diversity and capture the evolution of the whole species. We found that strains WB53 and HUN142 were misidentified and did not belong to the Clostridium beijerinckii species. Additionally, we filtered possibly misassembled genomes, and we used the remaining 237 high-quality genomes to define the pangenome of the whole species. By its functional annotation, we showed that the core genome contains genes responsible for basic metabolism, while the accessory genome has genes affecting final phenotype that may vary among different strains. We used the core genome to reconstruct the phylogeny of the species and showed its great diversity, which complicates the identification of particular strains, yet hides possibilities to reveal hitherto unreported phenotypic features and processes utilizable in biotechnology.
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Keywords
ABE, accessory genome, Butanol, core genome, IBE, pan genome
Subject
Suggested Citation
Sedlar K, Nykrynova M, Bezdicek M, Branska B, Lengerova M, Patakova P, Skutkova H. Diversity and Evolution of Clostridium beijerinckii and Complete Genome of the Type Strain DSM 791T. (2023). LAPSE:2023.5577
Author Affiliations
Sedlar K: Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 10, 616 00 Brno, Czech Republic
Nykrynova M: Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 10, 616 00 Brno, Czech Republic [ORCID]
Bezdicek M: Department of Internal Medicine−Hematology and Oncology, University Hospital Brno, Cernopolni 9, 613 00 Brno, Czech Republic; Department of Internal Medicine−Hematology and Oncology, Masaryk University, Jihlavska 20, 625 00 Brno, Czech Republic [ORCID]
Branska B: Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic
Lengerova M: Department of Internal Medicine−Hematology and Oncology, University Hospital Brno, Cernopolni 9, 613 00 Brno, Czech Republic
Patakova P: Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic
Skutkova H: Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 10, 616 00 Brno, Czech Republic [ORCID]
Nykrynova M: Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 10, 616 00 Brno, Czech Republic [ORCID]
Bezdicek M: Department of Internal Medicine−Hematology and Oncology, University Hospital Brno, Cernopolni 9, 613 00 Brno, Czech Republic; Department of Internal Medicine−Hematology and Oncology, Masaryk University, Jihlavska 20, 625 00 Brno, Czech Republic [ORCID]
Branska B: Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic
Lengerova M: Department of Internal Medicine−Hematology and Oncology, University Hospital Brno, Cernopolni 9, 613 00 Brno, Czech Republic
Patakova P: Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic
Skutkova H: Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno University of Technology, Technicka 10, 616 00 Brno, Czech Republic [ORCID]
Journal Name
Processes
Volume
9
Issue
7
First Page
1196
Year
2021
Publication Date
2021-07-10
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr9071196, Publication Type: Journal Article
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LAPSE:2023.5577
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doi:10.3390/pr9071196
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[v1] (Original Submission)
Feb 23, 2023
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Feb 23, 2023
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