LAPSE:2023.35103
Published Article
LAPSE:2023.35103
Hypoglycemic Potential, Antioxidant and Prebiotic Activity after Simulated Digestion of Combined Blueberry Pomace and Chia Seed Extracts
April 28, 2023
This study aimed to evaluate the hypoglycemic potential, antioxidant activity and prebiotic activity of a hydroalcoholic extract of blueberry pomace (BP), an aqueous extract of chia seeds (CS) and a novel combination of BP−CS extracts (BCM) for further use as ingredient of functional food. Spectrometric and HPLC analyses were used to characterize the total phenolic and flavonoid content and composition of BP, while CS was analyzed for total carbohydrate content. Data showed that the BCM mixture exerted an inhibition of α-amylase activity, which was 1.36 times higher than that of BP and 1.25 higher than CS extract. The mixture also showed better scavenging activity of free DPPH radicals than individual extracts, and had an IC50 value of 603.12 µg/mL. In vitro testing indicated that both serum- and colon-reaching products of simulated intestinal digestion of BCM presented the capacity to protect Caco-2 intestinal cells against oxidative stress by inhibition of reactive oxygen species production. In addition, the colon-reaching product of BCM digestion had the capacity to significantly (p < 0.05) stimulate the growth of Lactobacillus rhamnosus and Lactobacillus acidophilus, revealing a prebiotic potential. All these results indicated that improved biological activity of the novel combination of BP and CS extracts could be due to the synergistic action of constituents. The combination is recommended for further testing and the development of novel functional food for controlling type 2 diabetes and gastrointestinal conditions.
Record ID
Keywords
berry polyphenols, chia polysaccharides, gut microbiota, hypoglycemic activity, reactive oxygen species, simulated digestion
Suggested Citation
Mihai E, Negreanu-Pirjol BS, Craciunescu O, Ciucan T, Iosageanu A, Seciu-Grama AM, Prelipcean AM, Utoiu E, Coroiu V, Ghenea AM, Negreanu-Pirjol T. Hypoglycemic Potential, Antioxidant and Prebiotic Activity after Simulated Digestion of Combined Blueberry Pomace and Chia Seed Extracts. (2023). LAPSE:2023.35103
Author Affiliations
Mihai E: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Negreanu-Pirjol BS: Faculty of Pharmacy, “Ovidius” University of Constanta, Capitan Aviator Al. Serbanescu Street, No. 6, Campus, Corp C, 900470 Constanta, Romania
Craciunescu O: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania [ORCID]
Ciucan T: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Iosageanu A: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Seciu-Grama AM: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Prelipcean AM: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Utoiu E: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Coroiu V: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Ghenea AM: S.C. Santo Raphael Srl, Dimitrie Pompei Bvd., No. 3-5, 020335 Bucharest, Romania
Negreanu-Pirjol T: Faculty of Pharmacy, “Ovidius” University of Constanta, Capitan Aviator Al. Serbanescu Street, No. 6, Campus, Corp C, 900470 Constanta, Romania; Academy of Romanian Scientists, Ilfov Street, No. 3, 050044 Bucharest, Romania [ORCID]
Negreanu-Pirjol BS: Faculty of Pharmacy, “Ovidius” University of Constanta, Capitan Aviator Al. Serbanescu Street, No. 6, Campus, Corp C, 900470 Constanta, Romania
Craciunescu O: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania [ORCID]
Ciucan T: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Iosageanu A: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Seciu-Grama AM: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Prelipcean AM: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Utoiu E: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Coroiu V: National Institute of Research and Development for Biological Sciences, Department of Cellular and Molecular Biology, Splaiul Independentei, No. 296, 060031 Bucharest, Romania
Ghenea AM: S.C. Santo Raphael Srl, Dimitrie Pompei Bvd., No. 3-5, 020335 Bucharest, Romania
Negreanu-Pirjol T: Faculty of Pharmacy, “Ovidius” University of Constanta, Capitan Aviator Al. Serbanescu Street, No. 6, Campus, Corp C, 900470 Constanta, Romania; Academy of Romanian Scientists, Ilfov Street, No. 3, 050044 Bucharest, Romania [ORCID]
Journal Name
Processes
Volume
11
Issue
4
First Page
1025
Year
2023
Publication Date
2023-03-28
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr11041025, Publication Type: Journal Article
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LAPSE:2023.35103
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doi:10.3390/pr11041025
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Apr 28, 2023
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