The possibility of the application of an unmanned aerial vehicle (UAV) in search and rescue activities in a deep underground mine has been investigated. In the presented case study, a UAV is searching for a lost or injured human who is able to call for help but is not able to move or use any communication device. A UAV capturing acoustic data while flying through underground corridors is used. The acoustic signal is very noisy since during the flight the UAV contributes high-energetic emission. The main goal of the paper is to present an automatic signal processing procedure for detection of a specific sound (supposed to contain voice activity) in presence of heavy, time-varying noise from UAV. The proposed acoustic signal processing technique is based on time-frequency representation and Euclidean distance measurement between reference spectrum (UAV noise only) and captured data. As both the UAV and “injured” person were equipped with synchronized microphones during the experiment, validation has been performed. Two experiments carried out in lab conditions, as well as one in an underground mine, provided very satisfactory results.