LAPSE:2023.24994
Published Article
LAPSE:2023.24994
Culturing of Melanocytes from the Equine Hair Follicle Outer Root Sheath
Hanluo Li, Jule Kristin Michler, Alexander Bartella, Anna Katharina Sander, Sebastian Gaus, Sebastian Hahnel, Rüdiger Zimmerer, Jan-Christoph Simon, Vuk Savkovic, Bernd Lethaus
March 28, 2023
Hair follicles harbor a heterogeneous regenerative cell pool and represent a putative low-to-non-invasively available source of stem cells. We previously reported a technology for culturing human melanocytes from the hair follicle outer root sheath (ORS) for autologous pigmentation of tissue engineered skin equivalents. This study translated the ORS technology to horses. We de-veloped a culture of equine melanocytes from the ORS (eMORS) from equine forelock hair follicles cultured by means of an analogue human hair follicle-based in vitro methodology. The procedure was adjusted to equine physiology by addition of equine serum to the culture medium. The hair follicles were isolated by macerating forelock skin rests, enzymatically digested and subjected to air-medium-interface cultivation method. The procedure resulted in differentiated equine melanocytes, which exhibited typical morphology, presence of melanosomes, expression of cytoskeleton proteins vimentin, α-SMA, Sox2, S100ß and tyrosinase as well as tyrosinase activity followed by production of melanin. According to all assessed parameters, eMORS could be ranked as partially melanotic melanocytes. The results of the study offer an experimental base for further insight into hair follicle biology in equine and for comparative studies of hair follicles across different species.
Keywords
equine hair follicle, horse, melanocytes, outer root sheath
Subject
Suggested Citation
Li H, Michler JK, Bartella A, Sander AK, Gaus S, Hahnel S, Zimmerer R, Simon JC, Savkovic V, Lethaus B. Culturing of Melanocytes from the Equine Hair Follicle Outer Root Sheath. (2023). LAPSE:2023.24994
Author Affiliations
Li H: Department of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, Germany [ORCID]
Michler JK: Institute of Veterinary Anatomy, University of Leipzig, 04103 Leipzig, Germany [ORCID]
Bartella A: Department of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, Germany [ORCID]
Sander AK: Department of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, Germany
Gaus S: Department of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, Germany
Hahnel S: Polyclinic for Dental Prosthetics and Material Sciences, University Hospital Leipzig, 04103 Leipzig, Germany
Zimmerer R: Department of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, Germany
Simon JC: Clinic for Dermatology, Venereology and Allergology, University Hospital Leipzig, 04103 Leipzig, Germany
Savkovic V: Department of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, Germany
Lethaus B: Department of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig, 04103 Leipzig, Germany [ORCID]
Journal Name
Processes
Volume
9
Issue
1
First Page
pr9010177
Year
2021
Publication Date
2021-01-19
Published Version
ISSN
2227-9717
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PII: pr9010177, Publication Type: Journal Article
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LAPSE:2023.24994
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doi:10.3390/pr9010177
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Mar 28, 2023
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