LAPSE:2023.24873
Published Article
LAPSE:2023.24873
Ultrafiltration Method for Plasma Protein Binding Studies and Its Limitations
Camelia-Maria Toma, Silvia Imre, Camil-Eugen Vari, Daniela-Lucia Muntean, Amelia Tero-Vescan
March 28, 2023
Plasma protein binding plays a critical role in drug therapy, being a key part in the characterization of any compound. Among other methods, this process is largely studied by ultrafiltration based on its advantages. However, the method also has some limitations that could negatively influence the experimental results. The aim of this study was to underline key aspects regarding the limitations of the ultrafiltration method, and the potential ways to overcome them. The main limitations are given by the non-specific binding of the substances, the effect of the volume ratio obtained, and the need of a rigorous control of the experimental conditions, especially pH and temperature. This review presents a variety of methods that can hypothetically reduce the limitations, and concludes that ultrafiltration remains a reliable method for the study of protein binding. However, the methodology of the study should be carefully chosen.
Keywords
limitations, non-specific binding, plasma protein binding, ultrafiltration
Subject
Suggested Citation
Toma CM, Imre S, Vari CE, Muntean DL, Tero-Vescan A. Ultrafiltration Method for Plasma Protein Binding Studies and Its Limitations. (2023). LAPSE:2023.24873
Author Affiliations
Toma CM: Center of Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 38 Gheorghe Marinescu Street, RO-540142 Targu Mures, Romania; Doctoral School of Medicine and Pharmacy, I.
Imre S: Center of Advanced Medical and Pharmaceutical Research, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 38 Gheorghe Marinescu Street, RO-540142 Targu Mures, Romania; Doctoral School of Medicine and Pharmacy, I.
Vari CE: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 38 Gheorghe Marinescu Street, RO-540142 Targu Mures, Romania [ORCID]
Muntean DL: Department of Analytical Chemistry and Drug Analysis, Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 38 Gheorghe Marinescu Street, RO-540142 Targu Mures, Romania
Tero-Vescan A: Department of Chemistry and Medical Biochemistry, Faculty of Medicine in English, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 38 Gheorghe Marinescu Street, RO-540142 Targu Mures, Romania
Journal Name
Processes
Volume
9
Issue
2
First Page
382
Year
2021
Publication Date
2021-02-19
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr9020382, Publication Type: Review
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LAPSE:2023.24873
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doi:10.3390/pr9020382
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Mar 28, 2023
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CC BY 4.0
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