LAPSE:2021.0579
Published Article
LAPSE:2021.0579
Samnamul (Shoots of Aruncus dioicus) Inhibit Adipogenesis by Downregulating Adipocyte-Specific Transcription Factors in 3T3-L1 Adipocytes
Ji Yeon Lee, Jeong-Yong Park, Hyun Taek Seo, Hyun-A Seong, Yun-Jeong Ji, Seung Eun Lee, Kyung Hye Seo, Hyung Don Kim
June 29, 2021
Adipocyte-specific transcription factors and antioxidants are considered the best target of obesity. Aruncus dioicus var. kamtschaticus (A. dioicus, Samnamul) is easily available owing to edible and inexpensive. However, the anti-adipogenic effects of the underlying mechanism of A. dioicus extract (ADE) have not yet been reported. In the present study, we evaluate anti-adipogenic pathway in 3T3-L1 adipocytes, antioxidant activities and quantified phenolics using high-performance liquid chromatography of ADE. The results revealed ADE had reduced adipocyte differentiation (0.72-fold vs. MDI (media of differentiation) control), triglyceride (TG; 0.50-fold vs. MDI control, p < 0.001), and total cholesterol contents (0.77-fold vs. MDI control) by regulating adipocyte-specific transcription factors (C/EBPα, PPARγ, and SREBP1) and their downstream mRNA (AdipoQ, Ap2, SREBP1-c, and FAS) levels. Furthermore, ADE has higher total phenol and flavonoid contents and scavenging assay in the DPPH and ABTS+. In particularly, ADE contains chlorogenic acid (7.04 mg/kg), caffeic acid (20.14 mg/kg), ferulic acid (1.74 mg/kg), veratric acid (29.31 mg/kg), cinnamic acid (4.70 mg/kg), and quercetin (4.18 mg/kg). In conclusion, since these phenols, especially quercetin, in the ADE appear to reduce differentiation, TG and cholesterol content by regulating adipocyte-specific transcription factors in adipocytes, ADE has the potential to be developed into a new antioxidant and anti-obesity therapeutics.
Keywords
adipocytes, adipogenesis, Aruncus dioicus, lipogenesis, phenolics
Subject
Suggested Citation
Lee JY, Park JY, Seo HT, Seong HA, Ji YJ, Lee SE, Seo KH, Kim HD. Samnamul (Shoots of Aruncus dioicus) Inhibit Adipogenesis by Downregulating Adipocyte-Specific Transcription Factors in 3T3-L1 Adipocytes. (2021). LAPSE:2021.0579
Author Affiliations
Lee JY: Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Eumsung 27709, Korea; Department of Food Science and Biotechnology, Chungbuk National University, Chungbuk 28644, Korea
Park JY: Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Eumsung 27709, Korea
Seo HT: Wild Vegetables Research Institute, Gangwondo Agricultural Research and Extension Services, Pyeongchang 25300, Korea
Seong HA: Department of Biochemistry, School of Life Sciences, Chungbuk National University, Cheongju 28644, Korea
Ji YJ: Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Eumsung 27709, Korea [ORCID]
Lee SE: Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Eumsung 27709, Korea
Seo KH: Development of Horticultural Crop Research, National Institute of Horticultural & Herbal Science, RDA, Jeonju 55365, Korea
Kim HD: Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Eumsung 27709, Korea; Department of Biochemistry, School of Life Sciences, Chungbuk National University, Cheongju 28644, Korea [ORCID]
Journal Name
Processes
Volume
8
Issue
12
Article Number
E1576
Year
2020
Publication Date
2020-11-29
Published Version
ISSN
2227-9717
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PII: pr8121576, Publication Type: Journal Article
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LAPSE:2021.0579
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doi:10.3390/pr8121576
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Jun 29, 2021
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Jun 29, 2021
 
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Calvin Tsay
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