LAPSE:2021.0520
Published Article
LAPSE:2021.0520
Production of Protein Hydrolysate Containing Antioxidant and Angiotensin -I-Converting Enzyme (ACE) Inhibitory Activities from Tuna (Katsuwonus pelamis) Blood
Natthaphon Mongkonkamthorn, Yuwares Malila, Suthasinee Yarnpakdee, Sakunkhun Makkhun, Joe M. Regenstein, Sutee Wangtueai
June 10, 2021
Tuna blood (TB) was subjected to enzymatic hydrolysis. The effects of the relationship of hydrolysis time (30−180 min) and enzyme concentration (0.5−3.0% w/w protein) on the degree of hydrolysis (DH), yield, antioxidant and angiotensin-I-converting enzyme (ACE) inhibitory activities were determined. The response surface methodology (RSM) showed that TB hydrolysis’s optimum conditions were hydrolysis for 180 min and Alcalase, Neutrase or Flavourzyme at 2.81%, 2.89% or 2.87% w/w protein, respectively. The hydrolysates with good DH (40−46%), yield (3.5−4.6%), the IC50 of DPPH (0.8−1.6 mg/mL) and ABTS (1.0−1.4 mg/mL) radical scavenging activity, ferric reducing antioxidant power (FRAP) value (0.28−0.65 mmol FeSO4/g) and IC50 of ACE inhibitory activity (0.15−0.28 mg/mL) were obtained with those conditions. The TB hydrolysate using Neutrase (TBHN) was selected for characterization in terms of amino acid composition, peptide fractions and sensory properties. The essential, hydrophobic and hydrophilic amino acids in TBHN were ~40%, 60% and 20% of total amino acids, respectively. The fraction of molecular weight <1 kDa showed the highest antioxidant and ACE inhibitory activities. Fishiness and bitterness were the main sensory properties of TBHN. Fortification of TBHN in mango jelly at ≤ 0.5% (w/w) was accepted by consumers as like moderately to like slightly, while mango jelly showed strong antioxidant and ACE inhibitory activities. TBHN could be developed for natural antioxidants and antihypertensive peptides in food and functional products.
Keywords
ACE inhibitory activities, antioxidants, Katsuwonus pelamis, neutrase, protein hydrolysates, skipjack, tuna blood
Subject
Suggested Citation
Mongkonkamthorn N, Malila Y, Yarnpakdee S, Makkhun S, Regenstein JM, Wangtueai S. Production of Protein Hydrolysate Containing Antioxidant and Angiotensin -I-Converting Enzyme (ACE) Inhibitory Activities from Tuna (Katsuwonus pelamis) Blood. (2021). LAPSE:2021.0520
Author Affiliations
Mongkonkamthorn N: Faculty of Agro-industry, Chiang Mai University, Chiang Mai 50100, Thailand
Malila Y: National Center for Genetic Engineering and Biotechnology, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
Yarnpakdee S: Faculty of Agro-industry, Chiang Mai University, Chiang Mai 50100, Thailand
Makkhun S: Faculty of Agriculture and Natural Resources, University of Phayao, Phayao 56000, Thailand
Regenstein JM: Department of Food Science, College of Agriculture and Life Science, Cornell University, Ithaca, NY 14853-7201, USA [ORCID]
Wangtueai S: Faculty of Agro-industry, Chiang Mai University, Chiang Mai 50100, Thailand; College of Maritime Studies and Management, Chiang Mai University, Samut Sakhon 74000, Thailand [ORCID]
Journal Name
Processes
Volume
8
Issue
11
Article Number
E1518
Year
2020
Publication Date
2020-11-22
Published Version
ISSN
2227-9717
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Original Submission
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PII: pr8111518, Publication Type: Journal Article
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LAPSE:2021.0520
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doi:10.3390/pr8111518
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Jun 10, 2021
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Jun 10, 2021
 
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Calvin Tsay
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